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Production of sophorolipids by Candida bombicola grown on soy molasses as substrate*,**

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Abstract

Sophorolipids (SLs) were produced from Candida bombicola using soy molasses and oleic acid as co-substrates. The purified SLs were obtained at 21 g l−1 and were 97% in lactone form. The major SL constituent (81% relative abundance) of the product mixture contains an oleoyl chain. The surface properties of the SLs obtained from the soy molasses/oleic acid fermentation had minimum surface-tension values of 37 mN m−1 (pH 6) and 38 mN m−1 (pH 9), and critical micelle concentration values of 6 mg l−1 (pH 6) and 13 mg l−1 (pH 9).

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References

  • Asmer H-J, Lang S, Wagner F, Wray V (1988) Microbial production, structure elucidation and bioconversion of sophorose lipids. J. Am. Oil Chem. Soc. 65: 1460-1466.

    Google Scholar 

  • Daniel H-J, Reuss M, Syldatk C (1998) Production of sophorolipids in high concentration from deproteinized whey and rapeseed oil in a two stage fed batch process using Candida bombicolaATCC 22214 and Cryptococcus curvatusATCC 20509. Biotechnol. Lett. 20: 1153-1156.

    Article  Google Scholar 

  • Davila A-M, Marchal R, Vandecasteele J-P (1997) Sophorose lipid fermentation with differentiated substrate supply for growth and production phases. Appl. Microbiol. Biotechnol. 47: 496-501.

    Article  Google Scholar 

  • Guilmanov V, Ballistreri A, Impallomeni G, Gross RA (2002) Oxygen transfer rate and sophorose lipid production by Candida bombicola. Biotechnol. Bioeng. 77: 489-494.

    Article  PubMed  Google Scholar 

  • Hommel RK, Huse K (1993) Regulation of sophorose lipid production by Candida(Torulopsis) apicola. Biotechnol. Lett. 15:853-858.

    Article  Google Scholar 

  • Hymowitz T, Collins FI, Panczner J, Walker WM (1972) Relationship between the content of oil, protein, and sugar in soybean seed. Agron. J. 64: 613-616.

    Google Scholar 

  • Lynd LR, Wyman CE, Gerngross TU (1999) Biocommodity engineering. Biotechnol Prog 15: 777-793.

    PubMed  Google Scholar 

  • Montelongo J-L, Chassy BM, McCord JD (1993) Lactobacillus salivariusfor conversion of soy molasses into lactic acid. J. Food Sci. 58: 863-866.

    Google Scholar 

  • Nuñez A, Ashby R, Foglia TA, Solaiman DKY (2001) Analysis and characterization of sophorolipids by liquid chromatography with atmospheric pressure chemical ionization. Chromatographia 53:673-677.

    Google Scholar 

  • Otto RT, Daniel H-J, Pekin G, Müller-Dekker K, Fürstenberger G, Reuss M, Syldatk C (1999) Production of sophorolipids from whey. II. Product composition, surface active properties, cytotoxicity and stability against hydrolases by enzymatic treatment.Appl Microbiol Biotechnol 52: 495-501.

    PubMed  Google Scholar 

  • Qureshi N, Lolas A, Blaschek HP (2001) Soy molasses as fermentation substrate for production of butanol using Clostridium beijerinckiiBA101. J. Ind. Microbiol. Biotechnol. 26: 290-295.

    PubMed  Google Scholar 

  • Rau U, Hammen S, Heckmann R, Wray V, Lang S (2001) Sophorolipids: a source for novel compounds. Ind. Crop. Prod. 13:85-92.

    Article  Google Scholar 

  • Salunkhe DK, Chavan JK, Adsule RN, Kadam SS (1991) World Oilseed: Chemistry, Technology, and Utilization. New York: Van Nostrand Reinhold.

    Google Scholar 

  • Scott Jr TA, Melvin EH (1953) Determination of dextran with anthrone. Anal. Chem. 25: 1656-1661.

    Google Scholar 

  • Solaiman DKY, Ashby RD, Foglia TA, Nuñez A, Marmer WN (2002) Fermentation-based processes for the conversion of fats, oils and derivatives into biopolymers and biosurfactants. In: Cherry JP, Pavlath AE, eds. Proceedings of the 31st United States-Japan Cooperative Program in Natural Resources (UJNR). Wyndmoor, PA: Eastern Regional Research Center, ARS, USDA, pp. V1-V10.

    Google Scholar 

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Correspondence to Daniel K.Y. Solaiman.

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Solaiman, D.K., Ashby, R.D., Nuñez, A. et al. Production of sophorolipids by Candida bombicola grown on soy molasses as substrate*,** . Biotechnology Letters 26, 1241–1245 (2004). https://doi.org/10.1023/B:BILE.0000036605.80577.30

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  • DOI: https://doi.org/10.1023/B:BILE.0000036605.80577.30

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