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GRP94 reduces cell death in SH-SY5Y cells perturbated calcium homeostasis

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Abstract

The endoplasmic reticulum (ER) resident—94 kDa glucose-regulated protein (GRP94), plays a pivotal role in cell death due to ER stress. In our study expression of GRP94 was increased in human neuroblastoma SH-SY5Y cells due to exposure to calcium ionophore A23187. A23187-mediated cell death was associated with activation of the major cysteine proteases, caspase-3 and calpain. Pretreatment with adenovirus-mediated antisense GRP94 (AdGRP94AS) reduced viability of SH-SY5Y cells subjected to A23187 treatment compared with wild type cells or cells with adenovirus-mediated overexpression of GRP94 (AdGRP94S). These results indicated that suppression of GRP94 is associated with accelerated cell death. Moreover, expression of GRP94 suppressed A23187-induced cell death and stabilized calcium homeostasis.

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Authors and Affiliations

  1. Department of Anatomy and Neuroscience, Osaka University Graduate School of Medicine, Osaka, 565-0871

    Y. Bando, T. Katayama, T. Manabe & M. Tohyama

  2. Core Research for Evolutional Science and Technology, Japan Science and Technology (CREST JST), Saitama, 332-0012

    T. Katayama & M. Tohyama

  3. Division of Cardiovascular Surgery, Department of Surgery, Osaka University Graduate School of Medicine, Osaka, 565-0871, Japan

    A. N. Aleshin

Authors
  1. Y. Bando
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  2. T. Katayama
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  3. A. N. Aleshin
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  4. T. Manabe
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  5. M. Tohyama
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Correspondence to Y. Bando.

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Bando, Y., Katayama, T., Aleshin, A.N. et al. GRP94 reduces cell death in SH-SY5Y cells perturbated calcium homeostasis. Apoptosis 9, 501–508 (2004). https://doi.org/10.1023/B:APPT.0000031446.95532.ad

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  • DOI: https://doi.org/10.1023/B:APPT.0000031446.95532.ad

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