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Effect of Granulocyte Colony-Stimulating-Factor Administration on Tissue Regeneration Due to Thioacetamide-Induced Liver Injury in Rats

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Abstract

It has been shown recently that granulocytecolony-stimulating factor (G-CSF) accelerates andenhances the hepatocyte proliferative capacity ofpartially hepatectomized rats. In the present study, weinvestigated the effect of G-CSF administration in a ratmodel of liver injury and regeneration, induced bythioacetamide (TAA) injection. TAA (300 mg/kg bodyweight) was injected intraperitoneally in male Wistarrats, and this was followed by administration ofeither saline (group A) or G-CSF at a dose of 150μg/kg body weight (group B), 24 hr later. The animalswere killed at different time points after TAA treatment and the rate of tritiated thymidineincorporation into hepatic DNA, the activity of theenzyme thymidine kinase (EC 2.7.1.21) in the liver, andthe assessment of the mitotic index of hepatocytes, wereemployed to estimate liver regeneration. Theadministration of TAA caused severe hepatic injury,recognized histopathologically and by the raisedactivities of the serum hepatic enzymes aspartate andalanine aminotransferases. The hepatic injury, which peaked 36 hr afterTAA injection, was followed by a regenerative process ofhepatocytes presenting peaks at time points of 48 and 60hr (group A). The administration of G-CSF 24 hr after the injection of TAA (group B) causeda statistically significantly increase in the hepatocyteproliferation indices examined (P < 0.001), comparedto those found in group A at the same time points. It was concluded that G-CSFadministration enhanced the hepatocyte proliferativecapacity in this model of liver injury induced by TAAadministration.

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Authors
  1. Stamatios E. Theocharis
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  2. Alexandra P. Margeli
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  3. Christos N. Kittas
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Theocharis, S.E., Margeli, A.P. & Kittas, C.N. Effect of Granulocyte Colony-Stimulating-Factor Administration on Tissue Regeneration Due to Thioacetamide-Induced Liver Injury in Rats. Dig Dis Sci 44, 1990–1996 (1999). https://doi.org/10.1023/A:1026657931829

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