Abstract
1. We developed a simple and sensitive peripheral analgesic test in mice.
2. Substance P (SP) given into the planta (i.pl.) of the mouse hind limb produced a flexor response. The flexor response was dependent on SP doses (0.1–100 pmol, i.pl.). When SP (10 pmol) was given every 5 min, there were stable flexor responses. These nociceptive responses were completely abolished by CP-96,345, a neurokinin 1 receptor antagonist.
3. SP-induced responses were also blocked by several signal transduction-related compounds, such as tetrodotoxin, EGTA, and U73122, a selective phospholipase C inhibitor.
4. These findings suggest that SP depolarizes peripheral nerve endings, possibly through inositol trisphosphate (Ins P3)-gated Ca2+ influx, followed by induction of action potentials in the peripheral axons of primary afferent neurons.
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Inoue, M., Tokuyama, S., Nakayamada, H. et al. In Vivo Signal Transduction of Tetrodotoxin-Sensitive Nociceptive Responses by Substance P Given into the Planta of the Mouse Hind Limb. Cell Mol Neurobiol 18, 555–561 (1998). https://doi.org/10.1023/A:1026335611162
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DOI: https://doi.org/10.1023/A:1026335611162