Abstract
Three-dimensional protein NMR spectra can be obtained significantly faster than by traditional methods by a projection-reconstruction procedure related to X-ray tomography. First, two orthogonal projections are acquired in quick two-dimensional experiments with the evolution parameters t1 or t2 set to zero. These projections define a three-dimensional lattice; all cross-peaks must lie on this lattice but not all lattice points are occupied. A third experiment with t1 and t2 incremented simultaneously and in a fixed ratio, generates a projection onto a tilted plane and thus establishes the positions of all the cross-peaks unambiguously. This projection-reconstruction technique has been tested on the 500 MHz three-dimensional HNCO spectrum of ubiquitin.
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Aue, W.P., Bartholdi, E. and Ernst, R.R. (1976) J. Chem. Phys., 64, 2229.
Bracewell, R.N. (1956) Austr. J. Phys., 9, 198.
Brutscher, B., Morelle, N., Cordier, F. and Marion, D. (1995) J. Magn. Reson., B109, 338-242.
Chen, J., De Angelis, A.A., Mandelshtam, V.A. and Shaka, A.J. (2003) J. Magn. Reson., 162, 74-89.
Chen, J., Mandelshtam, V.A. and Shaka, A.J. (2000) J. Magn. Reson., 146, 363-368.
Ding, K. and Gronenborn, A. (2002) J. Magn. Reson., 156, 262-268.
Frydman, L., Lupulescu, A. and Scherf, T. (2003) J. Amer. Chem. Soc., 125, 9204-9217.
Frydman, L., Scherf, T. and Lupulescu, A. (2002) Proc. Natl. Acad. Sci. USA, 99, 15859-15862.
Hounsfield, G.N. (1973) Brit. J. Radiol., 46, 1016.
Jeener, J. (1971) Ampère International Summer School, Basko Polje, Yugoslavia.
Kay, L.E., Xu, G.Y. and Yamazaki, T.J. (1994) J. Magn. Reson., A109, 129-133.
Kim, S. and Szyperski, T. (2003) J. Am. Chem. Soc., 125, 1385-1393.
Kozminski, W. and Zhukov, I. (2003) J. Biomol. NMR, 26, 157-166.
Kupče, E. and Freeman, R. (2003a) J. Magn. Reson., 162, 300-310.
Kupče, E. and Freeman, R. (2003b) J. Magn. Reson., 163, 56-63.
Kupče, E. and Freeman, R. (2003c) J. Biomol. NMR, 25, 349-354.
McIntyre, L. and Freeman, R. (1989) J. Magn. Reson., 83, 649-635.
McIntyre, L. Wu, X-L. and Freeman, R. (1990) J. Magn. Reson., 87, 194-201.
Nagayama, K. Bachmann, P. Wüthrich, K. and Ernst, R.R. (1978) J. Magn. Reson., 31, 133.
Szyperski, T., Yeh, D.C., Sukumaran, D.K., Moseley, H.N.B. and Montelione, G.T. (2002) Proc. Natl. Acad. Sci. USA, 99, 8009-8014.
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Kupče, E., Freeman, R. Reconstruction of the three-dimensional NMR spectrum of a protein from a set of plane projections. J Biomol NMR 27, 383–387 (2003). https://doi.org/10.1023/A:1025819517642
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DOI: https://doi.org/10.1023/A:1025819517642