Skip to main content
SpringerLink
Log in

Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l−1 at the 200 l scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baumgartner P, Raemaekers RJM, Durieux A, Gatehouse A, Davies H, Taylor M (2002) Large-scale production, purification and characterisation of recombinant Phaeseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris. Prot. Expr. Purif. 26: 394–405.

    Google Scholar 

  • Holmquist M, Tessier DC, Cygler M (1997) High-level production of recombinant Geotrichum candidum lipases in the yeast Pichia pastoris. Prot. Expr. Purif. 11: 35–40.

    Google Scholar 

  • Longstaff M, Powell KS, Gatehouse JA, Raemaekers R, Nevell CA, Hamilton WDO (1998) Production and purification of active snowdrop lectin in Escherichia coli. Eur. J. Biochem. 252: 59–65.

    Google Scholar 

  • Raemaekers RJM, de Muro L, Gatehouse JA, Fordham-Skelton AP (1999) Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris. Eur. J. Biochem. 265: 394–403.

    Google Scholar 

  • Shibuya N, Goldstein IJ, Van Damme EJM, Peumans WJ (1988) Binding properties of a mannose-specific lectin from the snowdrop (Galanthus nivalis) bulb. J. Biol. Chem. 263: 728–734.

    Google Scholar 

  • Van Damme EJM, Goldstein IJ, Peumans WJ (1991) A comparative study of mannose-binding lectins from the Amaryllidaceae and Alliaceae. Phytochemistry (Oxf.) 30: 509–514.

    Google Scholar 

  • Van Damme EJM, Peumans W, Pustzai A, Bardocz S (1998) Handbook of Plant Lectins: Properties and Biomedical Applications Chichester, UK: John Wiley and Sons Ltd.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Quality, Health & Nutrition Group, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland

    Philippe Baumgartner, Karen Harper, Howard V. Davies & Mark A. Taylor

  2. Department Agricultural and Environmental Sciences, King George VI Building, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    Romaan J.M. Raemaekers & Angharad M.R. Gatehouse

  3. Unité de Biotechnologie, Institut Meurice, Bat. 2 Campus CERIA, Av. E Gryzon, 1070, Brussels, Belgium

    Alain Durieux

Authors
  1. Philippe Baumgartner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karen Harper
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Romaan J.M. Raemaekers
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alain Durieux
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Angharad M.R. Gatehouse
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Howard V. Davies
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mark A. Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mark A. Taylor.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baumgartner, P., Harper, K., Raemaekers, R.J. et al. Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris . Biotechnology Letters 25, 1281–1285 (2003). https://doi.org/10.1023/A:1025007901322

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1025007901322

Search

Navigation