Abstract
A plasmid pSDK-1 containing the Escherichia coli phosphofructokinase-1 gene (pfkA) was constructed, and transferred into Acidithiobacillus thiooxidans Tt-7 by conjugation. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium but the enzyme activity (18 U g−1) was lower than that in E. coli (K12: 86 U g−1; DF1010 carrying plasmid pSDK-1: 97 U g−1). In the presence of glucose, the Tt-7 transconjugant consumed glucose leading to a better growth yield.
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Tian, K.L., Lin, J.Q., Liu, X.M. et al. Conversion of an obligate autotrophic bacteria to heterotrophic growth: expression of a heterogeneous phosphofructokinase gene in the chemolithotroph Acidithiobacillus thiooxidans . Biotechnology Letters 25, 749–754 (2003). https://doi.org/10.1023/A:1023588921918
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DOI: https://doi.org/10.1023/A:1023588921918