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Use of a Medium Devoid of Any Human or Animal Compound (SMART2) for Embryo Culture in Intracytoplasmic Sperm Injection

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Abstract

Purpose: Our purpose wax to evaluate the efficiency of a medium, devoid of any human or animal compound and specially designed for early embryo development (from the zygote to the eight-cell stage), SMART2, in intracytoplasmic sperm injection (ICSI) and to compare it with a medium containing human serum albumin (EllioStep2).

Methods: Oocytes from 50 ICSI attempts were randomly placed, after sperm injection, into either SMART2 or EllioStep2. After a 48-hr incubation, the embryos were examined for quality scoring before transfer or freezing.

Results: The percentage of normally fertilized oocytes per intact oocytes was slightly higher using SMART2 (139/199 vs. 135/224, respectively, for SMART2 and EllioStep2: P < 0.05). The distribution of embryo scores and the percentage of embryos with a fair morphology (71/143 vs. 72/148, respectively, for SMART2 and EllioStep2; not significant) were identical in both media.

Conclusions: These data show that SMART2 medium can be successfully used for early embryo growth and, because it is devoid of any human or animal compound, offers better safety for patients than conventional media.

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REFERENCES

  1. Parinaud J, Monrozies X, Reme J-M, Sarramon M-F, Grandjean H, Pontonnier G: Randomized trial of two media used for in vitro fertilization. Eur J Gynecol Reprod Biol 1987;25:205–208

    Google Scholar 

  2. Staessen C, Van den Abbeel E, Janssenswillen C, Devroey P, Van Steirteghem AC: Controlled comparison of Earle's balanced salt solution with Ménézo B2 medium for human in-vitro fertilization performance. Hum Reprod 1994;9:1915–1919

    Google Scholar 

  3. Menezo Y, Testart J, Perrone D: Serum is not necessary in human in vitro fertilization, early embryo culture, and transfer. Fertil Steril 1984;42:750–755

    Google Scholar 

  4. Tam PP, Chan WY, Mao KR, Chiu T: A correlative study on the embryotrophic property of patient's serum and the outcome of in vitro fertilization of human oocytes. Fertil Steril 1987; 48:834–839

    Google Scholar 

  5. Miller KA, Pittaway DE, Deaton JL: The effect of serum from infertile women with endometriosis and early embryonic development in a murine in vitro fertilization model. Fertil Steril 1995;64:623–626

    Google Scholar 

  6. Dröge JH, Janssen LH, Wilting J: A comparative study of some physico-chemical properties of human serum albumin samples from different sources. Some physico-chemical properties of isionic human serum albumin solutions. Biochem Pharmacol 1982;31:3775–3779

    Google Scholar 

  7. Kane MT: Variability in different lots of commercial bovine serum albumin affects cell multiplication and hatching of rabbit blastocysts in culture. J Reprod Fertil 1983;69:555–558

    Google Scholar 

  8. Parinaud J: Packaging—California style. Fertil Steril 1997;68:385

    Google Scholar 

  9. Graham MC, Partridge AB, Lewis V, Phipps WR: A prospective comparison of synthetic serum substitute and human serum albumin in culture for in vitro fertilization-embryo transfer. Fertil Steril 1995;64:1036–1038

    Google Scholar 

  10. Dugan KJ, Shalika S, Smith RD, Padilla SL: Comparison of synthetic serum substitute and fetal cord serum as media supplements for in vitro fertilization: A prospective, randomized study. Fertil Steril 1997;67:166–168

    Google Scholar 

  11. Krone B, Lenz A, Heermann KH, Seifer M, Lu XY, Gerlich WH: Interaction between hepatitis B surface proteins and monomeric human serum albumin. Hepatology 1990;11:1050–1056

    Google Scholar 

  12. Stefas E, Rucheton M, Graafland H, Moynier M, Sompeyrac C, Bahraoui EM, Veas F: Human plasmatic apolipoprotein H binds human immunodeficiency virus type 1 and type 2 proteins. AIDS Res Hum Retrov 1997;13:97–104

    Google Scholar 

  13. Erstad BL: Viral infectivity of albumin and plasma fraction. Pharmacotherapy 1996;16:996–1001

    Google Scholar 

  14. Sakkas D, Urner F, Menezo Y, Leppens G: Effects of glucose and fructose on fertilization, cleavage, and viability of mouse embryos in vitro. Biol Reprod 1993;49:1288–1292

    Google Scholar 

  15. Bavister BD: Culture of preimplantation embryos: facts and artifacts. Hum Reprod Update 1995;1:91–148

    Google Scholar 

  16. Leese HJ: Metabolic control during preimplantation mammalian development. Hum Reprod Update 1995;1:63–72

    Google Scholar 

  17. Gardner DK, Lane M: Culture and selection of viable blastocysts: A feasible proposition for human IVF? Hum Reprod Update 1997;3:367–382

    Google Scholar 

  18. Parinaud J, Veiga A, Vieitez G, Busquets A, Milhet P, Calderon G, Richoilley G, Barri P: Randomized comparison of 3 media used for embryo culture after intracytoplasmic sperm injection. Hum Reprod 1998 (in press)

  19. Parinaud J, Mieusset R, Vieitez G, Labal B, Richoilley G: Influence of sperm parameters on embryo quality. Fertil Steril 1993;60:888–892

    Google Scholar 

  20. Palermo G, Joris H, Derde M-P, Camus M, Devroey P, Van Steirteghem AC: Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertil Steril 1993;59:826–835

    Google Scholar 

  21. Giorgetti C, Terriou P, Auquier P, Hans E, Spach J-L, Salzmann J, Roulier R: Embryo score to predict implantation after in-vitro fertilization: Based on 957 single embryo transfers. Hum Reprod 1995;10:2427–2431

    Google Scholar 

  22. Mahadevan MM, Miller MM, Moutos DM: Absence of glucose decreases human fertilization and sperm movement characteristics in vitro. Hum Reprod 1997;12:119–123

    Google Scholar 

  23. Conaghan J, Handyside AH, Winston RML, Leese HJ: Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J Reprod Fertil 1993;99:87–95

    Google Scholar 

  24. Barnett DK, Clayton MK, Kimura J, Bavister BD: Glucose and phosphate toxicity in hamster preimplantation embryos involves disruption of cellular organization, including distribution of active mitochondria. Mol Reprod Dev 1997;48:227–237

    Google Scholar 

  25. Truyen U, Parrish CR, Harder TC, Kaaden OR: There is nothing permanent except change. The emergence of new virus diseases. Vet Microbiol 1995;43:103–122

    Google Scholar 

  26. Ackerman SB, Swanson RJ, Stokes GK, Veeck LL: Culture of mouse preimplantation embryos as a quality control assay for human in vitro fertilization. Gamete Res 1984;9:145–152

    Google Scholar 

  27. Parinaud J, Reme J-M, Monrozies X, Favrin S, Sarramon M-F, Pontonnier G: Mouse system quality control is necessary before the use of new material for in vitro fertilization and embryo transfer. J In Vitro Fert Embryo Transfer 1987;4:56–58

    Google Scholar 

  28. Batt PA, Miller BG: Development of sheep embryos in vitro in a medium supplemented with different batches of serum albumin. Aust J Biol Sci 1988;41:371–376

    Google Scholar 

  29. McKiernan SH, Bavister BD: Different lots of bovine serum albumin inhibit or stimulate in vitro development of hamster embryos. In Vitro Cell Dev Biol 1992;28A:154–156

    Google Scholar 

  30. Nonogaki T, Noda Y, Goto Y, Kishi J, Mori T: Developmental blockage of mouse embryos caused by fatty acids. J Assist Reprod Genet 1994;11:482–488

    Google Scholar 

  31. Melsert R, Bos OJ, Van der Linden RF, Fisher MJ, Wilting J, Janssen LH, Hoogerbrugge JW, Rommerts FF: The stimulatory effect of albumin on luteinizing hormone-stimulated Leydig cell steroid production depends on its fatty acid content and correlates with conformational changes. Mol Cell Endocrinol 1991;82:23–32

    Google Scholar 

  32. Parinaud J, Milhet P, Vieitez G, Richoilley G: Human sperm capacitation and in vitro fertilization in a chemically defined and protein-free medium SMART1. Hum Reprod 1998;13:2579–2582

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Fécondation In Vitro, CHU La Grave, Toulouse Cedex, France

    Jean Parinaud, Pierrette Milhet, Gérard Vieitez & Gérard Richoilley

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  1. Jean Parinaud
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  2. Pierrette Milhet
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  3. Gérard Vieitez
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  4. Gérard Richoilley
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Parinaud, J., Milhet, P., Vieitez, G. et al. Use of a Medium Devoid of Any Human or Animal Compound (SMART2) for Embryo Culture in Intracytoplasmic Sperm Injection. J Assist Reprod Genet 16, 13–16 (1999). https://doi.org/10.1023/A:1022537410966

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  • DOI: https://doi.org/10.1023/A:1022537410966

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