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The Lymantria dispar Nucleopolyhedrovirus Contains the Capsid-Associated p24 Protein Gene

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Abstract

During the course of investigations on a wild-type strain of Lymantria dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV), a region of the viral genome was analyzed and found to contain 697 bp that is lacking in the sequenced strain (5–6) of LdMNPV (Kuzio et al., Virology 253, 17–34, 1999). The sequenced strain of LdMNPV contains a mutation in the 25 K few polyhedra (FP) gene, and exhibits the phenotype of a FP mutant. The additional sequence was located at approximately 81.4 map units within the viral genome, and was found in 10 different wild-type LdMNPV genotypic variants analyzed. Since the additional sequence was found in all wild-type virus strains analyzed, this sequence should be included in the representative LdMNPV genome. Sequence analysis of the genomic region containing the additional sequences revealed the presence of a homologue of the Autographa californica MNPV capsid-associated p24 gene (ORF 129). This gene, absent in LdMNPV isolate 5–6, is also present in the Orgyia pseudotsugata MNPV, Bombyx mori NPV, Spodoptera exigua MNPV, S. litura MNPV, Mamestra configurata MNPV, Helicoverpa armigera SNPV, H. zea SNPV, Buzura suppressaria SNPV, Xestia c-nigrum granulovirus, Plutella xylostella GV, and Cydia pomonella GV.

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Authors and Affiliations

  1. USDA Forest Service, 359 Main Road, Delaware, OH, 43015, USA

    James M. Slavicek & Nancy Hayes-Plazolles

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  1. James M. Slavicek
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  2. Nancy Hayes-Plazolles
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Correspondence to James M. Slavicek.

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Slavicek, J.M., Hayes-Plazolles, N. The Lymantria dispar Nucleopolyhedrovirus Contains the Capsid-Associated p24 Protein Gene. Virus Genes 26, 15–18 (2003). https://doi.org/10.1023/A:1022317819378

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