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Genetic Variation in Some Populations of the Golden-Striped Salamander, Chioglossa lusitanica (Amphibia: Urodela), in Portugal

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Abstract

Genetic variation in the golden-striped salamander (Chioglossa lusitanica) was assessed in 231 individuals from four Portuguese populations by means of horizontal starch gel electrophoresis and isoelectric focusing. Three of 19 enzyme systems, representing 21 presumptive loci, were found to be polymorphic: phosphoglucomutase 1 (PGM1), peptidase B (PEPB), and peptidase D (PEPD). The observed average heterozygosity in Chioglossa lusitanica (0.027) is significantly lower than that observed for other amphibians, either urodeles or salamandrids. Differences in allele frequencies and the presence of private alleles are indicative of a high degree of population differentiation. PEPD, in particular, seems to be a diagnostic locus separating the southernmost population studied from the others.

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Authors and Affiliations

  1. Departamento de Zoologia e Antropologia, Faculdade de Ciências, Universidade do Porto, Praça Gomes Teixeira, 4050, Porto, Portugal

    João Alexandrino & Nuno Ferrand

  2. Centro de Estudos em Ciência Animal, ICETA, Campus Agrário de Vairão, Rua do Monte (Crasto)—Vairão, 4480, Vila do Conde, Portugal

    João Alexandrino & Nuno Ferrand

  3. School of Biological Sciences, University of Wales, Brambell Building, Bangor, LL57 2UW, United Kingdom

    J. W. Arntzen

Authors
  1. João Alexandrino
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  2. Nuno Ferrand
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  3. J. W. Arntzen
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Alexandrino, J., Ferrand, N. & Arntzen, J.W. Genetic Variation in Some Populations of the Golden-Striped Salamander, Chioglossa lusitanica (Amphibia: Urodela), in Portugal. Biochem Genet 35, 371–381 (1997). https://doi.org/10.1023/A:1022281616801

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