Abstract
We have analyzed the distribution of aldolase A and C mRNAs and proteins in various areas of the human brain using Northern blot analyses and immunohistochemistry. Aldolase A mRNA expression was higher than aldolase C mRNA expression in all areas of the brain examined. Aldolase C mRNA expression was highest in the cerebellum. Aldolase C protein was present in well-delimited regions of the CNS, and was distributed in stripes in the Purkinje cell layer of the cerebellum, in the inferior olives and in the sensory neurons of the posterior horn of the spinal cord. The novel finding of aldolase C in well-delimited cell compartments of the human cerebellum and in several other areas of the CNS lends weight to the hypothesis that this protein exerts other functions (e.g. sensory transmission) besides those characteristic of a glycolytic enzyme.
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References
Ahn, A. H., Dziennis, S., Hawkes, R. & Herrup, K. (1994) The cloning of zebrin II reveals its identity with aldolase C. Development 120, 2081–2090.
Ali, M., rellos, P. & Cox, T. (1998) Hereditary fructose intolerance. Journal of Medical Genetics 35, 353–365.
CaffÈ, A. R., von Schantz, A., SzÉl, A., Voogd, J. & van Veen, T. (1994) Distribution of Purkinje cell-specific zebrin II/aldolase C immunoreactivity in the mouse, rat, rabbit and human retina. Journal of Comparative Neurology 348, 291–297.
Gitzelmann, R., Steinmann, B. & van der Berghe, G. (2001) Disorders of fructose metabolism. In The Metabolic and Molecular Bases of Inherited Disease (edited by Scriver, C. R., Beaudet, A. L., Sly, W. S. & Walle, D.) pp. 1489–1520. New York: McGraw-Hill.
Hamperl, H. (1977) Autopsia (translated by Minicucci, F.) Idelson: Napoli (Translation from: Leichenögfnung befund und diagnose).
Hawkes, R. & Herrup, K. (1995) Aldolase C/zebrin II and the regionalization of the cerebellum. Journal of Molecular Neuroscience 6, 147–158.
Inagaki, H., Haimoto, H., Hosoda, S. & Kato, K. (1988) Aldolase C is localized in neuroendocrine cells. Experientia 44, 749–751.
Izzo, P., Costanzo, P., Lupo, A., Rippa, E., Borghese, A. M., Paolella, G. & Salvatore, F. (1987) A new humans pecies of aldolase A mRNA from fibroblasts. European Journal of Biochemistry 164, 9–13.
Kitajima, Y., Matsuhashi, S., Nishida, H., Takasaki, Y., Takahashi, I., Hisatsugu, T. & Hori, K. (1991) Monoclonal antibodies recognizing amino-terminal and carboxy-terminal regions of human aldolase A: Probes to detect conformational changes of the enzyme. Journal of Biochemistry 109, 544–550.
Laughton, J. D., Charnay, Y., Belloir, B., Pellerin, L., Magistretti, P. J. & Bouras, C. (2000) Differential messenger RNA distribution of lactate dehydrogenase LDH-1 and LDH-5 isoforms in the rat brain. Neuroscience 96, 619–625.
Mukai, T., Yatsuki, H., Masuko, S., Arai, Y., Joh, K. & Hori, K. (1991) The structure of the brainspecific rat aldolase C gene and its regional expression. Biochemical and Biophysical Research Communications 174, 1035–1042.
Paolella, G., Buono, P., Mancini, F. P., Izzo, P. & Salvatore, F. (1990) Structure and expression of mouse aldolase genes. Brain-specific aldolase C amino acid sequence is closely related to aldolase A. European Journal of Biochemistry 156, 229–235.
Popovici, T., Berwald-Netter, Y., Vibert, M., Kahn, A. & Skala, H. (1990) Localization of aldolase C mRNA in brain cells. FEBS Letters 268, 189–193.
Salvatore, F., Izzo, P. & Paolella, G. (1986) Aldolase gene protein families: structure, expression and pathophysiology. In Human Genes and Diseases (edited by Blasi, F.) pp. 611–665. New York: JohnWiley and Sons.
Santamaria, R., Esposito, G., Vitagliano, L., Race, V., Paglionico, I., Zancan, L., Zagari, A. & Salvatore, F. (2000) Functional and molecular modelling studies of two hereditary fructose intolerance-causing mutations at arginine 303 in human liver aldolase. Biochemical Journal 350, 823–828.
Santamaria, R., Vitagliano, L., Tamasi, S., Izzo, P., Zancan, L., Zagari, A. & Salvatore, F. (1999) Novel six-nucleotide deletion in the hepatic fructose-1,6-bisphosphate aldolase gene in a patient with hereditary fructose intolerance and enzyme structure-function implications. European Journal of Human Genetics 7, 409–414.
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Buono, P., D'Armiento, F.P., Terzi, G. et al. Differential distribution of aldolase A and C in the human central nervous system. J Neurocytol 30, 957–965 (2001). https://doi.org/10.1023/A:1021828421792
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DOI: https://doi.org/10.1023/A:1021828421792