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Purification and characterisation of NAD+-dependent xylitol dehydrogenase from Fusarium oxysporum

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Abstract

An NAD+-dependent xylitol dehydrogenase (XDH) from Fusarium oxysporum, a key enzyme in the conversion of xylose to ethanol, was purified to homogeneity and characterised. It was homodimeric with a subunit of M r 48 000, and pI 3.6. It was optimally active at 45 °C and pH 9–10. It was fully stable at pH 6–7 for 24 h and 30 °C. K m values for d-xylitol and NAD+ were 94 mM and 0.14 mM, respectively. Mn2+ at 10 mM increased XDH activity 2-fold and Cu2+ at 10 mM inhibited activity completely.

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References

  • Bolen PL, Roth KA, Freer SN (1986) Affinity purifications of aldose reductase and xylitol dehydrogenase from the xylose fermenting yeast Pachysolen tannophilus. Appl. Environ. Microbiol. 52: 660–664.

    Google Scholar 

  • Bruinenberg PM (1983) The role of redox balances in the anaerobic fermentation of xylose by yeasts. Eur. J. Appl. Microbiol. Biotechnol. 18: 287–292.

    Google Scholar 

  • Chiang C, Knight SG (1960) Metabolism of D-xylose by moulds. Nature 188: 79–81.

    Google Scholar 

  • Christakopoulos P, Kekos D, Macris BJ (1989) Direct fermentation of cellulose to ethanol by Fusarium oxysporum. Enzyme Microb. Technol. 11: 236–239.

    Google Scholar 

  • Christakopoulos P, Kekos D, Macris BJ, Claeyssens M, Bhat MK (1995) Purification and characterisation of a non-randomly acting endo-1,4-?-D-glucanase from the culture filtrates of Fusarium oxysporum. Arch. Biochem. Biophys. 316: 428–433.

    Google Scholar 

  • Christakopoulos P, Nerinckx B, Kekos D, Macris BJ, Claeyssens M (1996) Purification and characterization of two low molecular mass alkaline xylanases from Fusarium oxysporum F3. J. Biotechnol. 51: 181–189.

    Google Scholar 

  • Christakopoulos P, Nerinckx B, Kekos D, Macris BJ, Claeyssens M (1997) The alkaline xylanase III from Fusarium oxysporum belongs to Family F. Carbohydr. Res. 302: 191–195.

    Google Scholar 

  • du Preez JC (1994) Process parameters and environmental factors affecting D-xylose fermentation by yeasts. Enzyme Microb. Technol. 16: 944–956.

    Google Scholar 

  • Morimoto S, Matsuo M, Azuma K, Sinskey AJ (1986) Purification and properties of D-xylulose reductase from Pachysolen tannophilus. J. Ferment. Technol. 64: 219–225.

    Google Scholar 

  • Panagiotou G, Kekos D, Macris BJ, Christakopoulos P (2002) The influence of aeration and external electron acceptors on xylose fermentation by Fusarium oxysporum. In: Proceedings of the ESBES-4 Symposium, August 8–31, Delft, pp. 92. ISBN 9090160132.

  • Phadrate SU, Rawat UB, Rao MB (1997) Purification and characterization of xylitol dehydrogenase from Neurospora crassa. FEMS Lett. 146: 79–83.

    Google Scholar 

  • von Sivers M, Zacchi G (1995) A techno-economical comparison of three processes for the production of ethanol from pine. Biores. Technol. 51: 43–52.

    Google Scholar 

  • Yang VW, Jeffries TW (1990) Purification and properties of xylitol dehydrogenase from the xylose-fermenting yeast Candida shehatae. Appl. Biochem. Biotechnol. 26: 197–206.

    Google Scholar 

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Authors and Affiliations

  1. Biotechnology Laboratory, Department of Chemical Engineering, National Technical University of Athens, Zografou Campus, Athens, 157 80, Greece

    G. Panagiotou, D. Kekos, B.J. Macris & P. Christakopoulos

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  1. G. Panagiotou
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  2. D. Kekos
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  3. B.J. Macris
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  4. P. Christakopoulos
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Panagiotou, G., Kekos, D., Macris, B. et al. Purification and characterisation of NAD+-dependent xylitol dehydrogenase from Fusarium oxysporum . Biotechnology Letters 24, 2089–2092 (2002). https://doi.org/10.1023/A:1021317614948

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  • DOI: https://doi.org/10.1023/A:1021317614948

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