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Production of hexanal from hydrolyzed sunflower oil by lipoxygenase and hydroperoxid lyase enzymes

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Abstract

Hexanal was produced from hydrolyzed sunflower oil in two steps: 1) 13-hydroperoxy-9-(Z),11(E)-octadecadienoic acid (13-HPOD) was formed from linoleic acid (100 mM) by soybean lipoxygenase-1 isoenzyme (Lox-1) with O2, the reaction resulted 68.7 mM 13-HPOD with a yield of 72%. 2) 13-HPOD (15 mM) was cleaved by spinach leaf hydroperoxide lyase resulting 8.2 mM hexanal (54% yield). Hexanal was isolated from the reaction mixture by repeated steam distillation.

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Authors and Affiliations

  1. Research Institute of Chemical and Process Engineering, University of Kaposvár, P.O. Box 125, Veszprém, Hungary, H-8201

    Judit Sz. Márczy, Ágnes Sz. Németh & Béla Szajáni

  2. Aroma Bázis Ltd., P.O. Box 32, Kistarcsa, Hungary, H-2143

    Zsuzsanna Samu & Ádám Háger-Veress

Authors
  1. Judit Sz. Márczy
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  2. Ágnes Sz. Németh
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  3. Zsuzsanna Samu
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  4. Ádám Háger-Veress
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  5. Béla Szajáni
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Márczy, J.S., Németh, Á.S., Samu, Z. et al. Production of hexanal from hydrolyzed sunflower oil by lipoxygenase and hydroperoxid lyase enzymes. Biotechnology Letters 24, 1673–1675 (2002). https://doi.org/10.1023/A:1020657618363

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  • DOI: https://doi.org/10.1023/A:1020657618363

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