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Alterations of Lymphocyte Populations in Lymph Nodes But Not in Spleen During the Latency Period of Adjuvant Arthritis

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Abstract

The aim of this study was to analyze potential imbalances in lymphocyte populations from regional lymph nodes (LN) and spleen occurring before the development of the outer inflammation of adjuvant arthritis (AA). Percentages and absolute numbers of CD5+, CD4+, CD8+, Ig+, I-A+, NKR-P1+ and TCRγδ+ cells were determined. No differences in percentages of γδ T or NK cells were found either in LN or spleen, thus ruling out an important role of these minor subpopulations in these early stages of AA. While no significant lymphocyte imbalances were observed in spleen, an increase in the percentage of B lymphocytes was found in regional LN. Moreover, a high proliferation of CD8+ cells was observed when measuring absolute numbers of LN lymphocytes, thus producing an imbalance in the CD4/CD8 ratio at very early stages of the inflammatory process. These findings suggest a role for CD8+ and B lymphocytes in the latency period of AA at the LN level. Our results indicate a primary role for lymph nodes in initiating the inflammation of AA, whereas cells from the spleen probably play a secondary role.

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REFERENCES

  1. Taurog, J. D., G. P. Sandberg, and M. L. Mahowald. 1983. The cellular basis of adjuvant arthritis. II. Characterization of the cells mediating passive transfers. Cell. Immunol. 80:198–204.

    PubMed  Google Scholar 

  2. Waksman, B. H., and C. Wennersten. 1963. Passive transfer of adjuvant arthritis in rats with living lymphoid cells of sensitized donors. Int. Arch. Allergy Appl. Immunol. 23:129–139.

    PubMed  Google Scholar 

  3. Billingham, M. E. J., C. Hicks, and S. Carney. 1990. Monoclonal antibodies and arthritis. Agents Actions. 29:77–87.

    PubMed  Google Scholar 

  4. Yoshino, S., L. G. Cleland, G. Mayrhofer, R. R. Brown, and J. H. Schwab. 1991. Prevention of chronic erosive streptococcal cell wall-induced arthritis in rats by treatment with a monoclonal antibody against the T cell antigen receptor αβ. J. Immunol. 146:4187–4189.

    PubMed  Google Scholar 

  5. Yoshino, S., and L. G. Cleland. 1992. Depletion of α/β T cells by a monoclonal antibody against the α/β T cell receptor suppresses established adjuvant arthritis, but not established collagen-induced arthritis in rats. J. Exp. Med. 175:907–915.

    PubMed  Google Scholar 

  6. PelegrÍ, C., M. P. Morante, C. Castellote, M. Castell, and A. Franch. 1995. Administration of a nondepleting anti-CD4 monoclonal antibody (W3/25) prevents adjuvant arthritis, even upon rechallenge: parallel administration of a depleting anti-CD8 monoclonal antibody (OX8) does not modify the effect of W3/25. Cell. Immunol. 165:177–182.

    PubMed  Google Scholar 

  7. PelegrÍ, C., M. P. Morante, C. Castellote, A. Franch, and M. Castell. 1996. Treatment with an anti-CD4 monoclonal antibody strongly ameliorates established rat adjuvant arthritis. Clin. Exp. Immunol. 103:273–278.

    PubMed  Google Scholar 

  8. Spargo, L. D. J., J. S. Hawkes, L. G. Cleland, and G. Mayrhofer. 1996. Recruitment of lymphoblasts derived from peripheral and intestinal lymph to synovium and other tissues in normal rats and rats with adjuvant arthritis. J. Immunol. 157:5198–5207.

    PubMed  Google Scholar 

  9. Newbould, B. B. 1964. Lymphatic drainage and adjuvant induced arthritis in rats. Brit. J. Exp. Pathol. 45:375–383.

    Google Scholar 

  10. Van den Eertwegh, A. J. M., W. J. A. Boersma, and E. Claassen. 1992. Immunological functions and in vivo cell-cell interactions of T cells in the spleen. Crit. Rev. Immunol. 11:337–380.

    PubMed  Google Scholar 

  11. Franch, A., C. Castellote, and M. Castell. 1994. Blood lymphocyte subsets in rats with adjuvant arthritis. Ann. Rheum. Dis. 53:461–466.

    PubMed  Google Scholar 

  12. Davidson, W. F., and C. R. Parish. 1975. A procedure for removing red cells and dead cells from lymphoid cell suspensions. J. Immunol. Methods 7:291–300.

    PubMed  Google Scholar 

  13. Dallman, M. J., M. L. Thomas, and J. R. Green. 1984. MRC OX-19: A monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur. J. Immunol. 14:260–267.

    PubMed  Google Scholar 

  14. White, R. A. H., D. W. Mason, A. F. Williams, G. Galfre, and C. Milstein. 1978. T-lymphocyte heterogeneity in the rat: Separation of functional subpopulations using a monoclonal antibody. J. Exp. Med. 148:664–673.

    PubMed  Google Scholar 

  15. Brideau, R. J., P. B. Carter, W. R. McMaster, M. W. Mason, and A. F. Williams. 1980. Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur. J. Immunol. 10:609–615.

    PubMed  Google Scholar 

  16. McMaster, W. R., and A. F. Williams. 1979. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur. J. Immunol. 9:426–433.

    PubMed  Google Scholar 

  17. Chambers, W. H., N. L. Vujanovic, A. B. Deleo, M. W. Olszowy, R. B. Herberman, and J. C. Hiserodt. 1989. Monoclonal antibody to a triggering structure expressed on rat natural killer cells and adherent lymphokine-activated killer cells. J. Exp. Med. 169:1373–1389.

    PubMed  Google Scholar 

  18. KÜhnlein, P., J. H. Park, T. Herrmann, A. Elbe, and T. HÜnig. 1994. Identification and characterization of rat γ/δ T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the γ/δ T cell receptor. J. Immunol. 153:979–986.

    PubMed  Google Scholar 

  19. Paulus, H. E., H. I. Machleder, S. Levine, D. T. Y. Yu, and N. S. MacDonald. 1977. Lymphocyte involvement in rheumatoid arthritis: Studies during thoracic duct drainage. Arthritis Rheum. 20:1249–1262.

    PubMed  Google Scholar 

  20. Panayi, G. S. 1992. The immunopathogenesis of rheumatoid arthritis. Clin. Exp. Immunol. 10:305–307.

    Google Scholar 

  21. Holoshitz, J., Y. Naparstek, A. Ben-Nun, and I. R. Cohen. 1983. Lines of T lymphocytes induce or vaccinate against autoimmune arthritis. Science. 219:56–58.

    PubMed  Google Scholar 

  22. PelegrÍ, C., A. Franch, C. Castellote, and M. Castell. 1995. Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis. J. Rheumatol. 22:124–132.

    PubMed  Google Scholar 

  23. Wooley, P. H., and J. D. Whalen. 1991. Pristane-induced arthritis in mice. III. Lymphocyte phenotypic and functional abnormalities precede the development of pristane-induced arthritis. Cell. Immunol. 138:251–259.

    PubMed  Google Scholar 

  24. Bersani-Amado, C. A., A. J. da S. Duarte, M. M. Tanji, M. Cianga, and Jancar S. 1990. Comparative study of adjuvant induced arthritis in susceptible and resistant strains of rats. III. Analysis of lymphocyte subpopulations. J. Rheumatol. 17:153–158.

    PubMed  Google Scholar 

  25. Banerjee, S., C. Webber, and A. R. Poole. 1992. The induction of arthritis in mice by the cartilage proteoglycan aggrecant: Roles of CD4+ and CD8+ T cells. Cell. Immunol. 144:347–357.

    PubMed  Google Scholar 

  26. BuzÁs, E. I., K. Mikecz, F. R. Brennan, and T. T. Glant. 1994. Mediators and autopathogenic effector cells in proteoglycan-induced arthritic and clinically asymptomatic BALB/c mice. Cell. Immunol. 158:292–304.

    PubMed  Google Scholar 

  27. Franch, A., S. Cassany, C. Castellote, and M. Castell. 1994. Time course of antibodies against IgG and type II collagen in adjuvant arthritis. Role of Mycobacteria administration in antibody production. Immunobiol. 190:93–104.

    Google Scholar 

  28. Ziesenis, A., B. RÖllinger, B. Franz, S. Hart, M. Hadam, and W. Leibold. 1992. Changes in rat leukocyte populations in peripheral blood, spleen, lymph nodes, and synovia during Erysipelas bacteria-induced polyarthritis. J. Exp. Anim. Sci. 35:2–15.

    PubMed  Google Scholar 

  29. Sugawara, T., M. Miyamoto, S. Takada, M. Nomura, and M. Kato. 1995. Alteration of spleen lymphocyte populations in rats with arthritis induced by muramyl dipeptide analogue or complete adjuvant. Int. J. Tiss. Reac. 17:5–13.

    Google Scholar 

  30. Tsai, C-L., and T-K. Liu. 1992. Lymphocyte subsets and mitogen induced proliferation in adjuvant arthritis. Part III. Numbers and functions of T lymphocytes in spleen. Tohoku J. Exp. Med. 166:289–296.

    PubMed  Google Scholar 

  31. Peterman, G. M., C. Spencer, A. I. Sperling, and J. A. Bluestone. 1993. Role of γδ T cells in murine collagen-induced arthritis. J. Immunol. 151:6546–6558.

    PubMed  Google Scholar 

  32. O'Brien, R. L., M. P. Happ, A. Dallas, E. Palmer, R. Kubo, and W. K. Born. 1989. Stimulation of a major subset of lymphocytes expressing T cell receptor γδ by an antigen derived from Mycobacterium tuberculosis. Cell. 57:667–674.

    PubMed  Google Scholar 

  33. PelegrÍ, C., P. KÜhnlein, E. Buchner, C. B. Schmidt, A. Franch, M. Castell, T. HÜnig, F. Emmrich, and R. W. Kinne. 1996. Depletion of γ/δ T cells does not prevent or ameliorate, but rather aggravates, rat adjuvant arthritis. Arthritis Rheum. 39:204–215.

    PubMed  Google Scholar 

  34. Torres-Nagel, N., E. Kraus, M. H. Brown, G. Tieffenthaler, R. Mitnacht, A. F. Williams, and T. HÜnig. 1992. Differential thymus dependence of rat CD8 isoform expression. Eur. J. Immunol. 22:2841–2848.

    PubMed  Google Scholar 

  35. Kummer, J. A., P. P. Tak, B. M. N. Brinkman, A. A. G. van Tilborg, A. M. Kamp, C. L. Verweii, M. R. Daha, A. E. Meinders, C. E. Hack, and F. C. Breedveld. 1994. Expression of granzymes A and B in synovial tissue from patients with rheumatoid arthritis and osteoarthritis. Clin. Immunol. Immunopathol. 73:88–95.

    PubMed  Google Scholar 

  36. Tak, P. P., J. A. Kummer, C. E. Hack, M. R. Daha, T. J. M. Smeets, G. W. Erkelens, A. E. Meinders, P. M. Kluin, and F. C. Breedveld. 1994. Granzyme-positive cytotoxic cells are specifically increased in early rheumatoid synovial tissue. Arthritis Rheum. 37:1735–1743.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department de Fisiologia-Divisió IV, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain

    Marta Rodríguez-Palmero, Carme Pelegrí, Maria José Ferri, Margarida Castell, Àngels Franch & Cristina Castellote

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  1. Marta Rodríguez-Palmero
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  2. Carme Pelegrí
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  3. Maria José Ferri
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  4. Margarida Castell
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  5. Àngels Franch
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  6. Cristina Castellote
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Rodríguez-Palmero, M., Pelegrí, C., Ferri, M.J. et al. Alterations of Lymphocyte Populations in Lymph Nodes But Not in Spleen During the Latency Period of Adjuvant Arthritis. Inflammation 23, 153–165 (1999). https://doi.org/10.1023/A:1020293012793

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