Abstract
A glucose-based wastewater was efficiently degradaded by acidogenic bacteria, with a glucose removal efficiency close to 90%, and although a distinctive granular structure could not be observed, fluffy conglomerates developed in an Upflow Anaerobic Sludge Blanket (UASB) reactor. Subsequently, the pre-acidification of the wastewater promoted the granulation process. An enrichment in methanogenic bacteria was observed on the microscope and was confirmed by an increase in the specific methanogenic activity from 0.1 up to 0.5kgCOD/kgVSS.day. Such dynamics of microbial communities was also verified through changes in the polysaccharide and protein content, as well as in the electrophoretic mobility of the biomass.
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References
American Public Health Association (1989). Standard Methods for the Examination of Water and Wastewater. 17th ed., APHA, AWWA, WPCF. Washington DC, USA.
Alibhai, K.R.K. and Forster C.F. (1986). Enzyme Microb. Technol., 8, 601–606.
Alphenaar, A. (1994). PhD Thesis, University of Wageningen, Wageningen, The Netherlands.
Dolfing, J., Griffioen, A., Neerven, A.R.W. and Zevenhuizen, L.P. (1985). Can. J. Microb., 31, 744–750.
Dolfing, J. and Mulder, J.W. (1985). Appl. Env. Microbiol. 49, 1142–1145.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956). Anal. Chem., 28, 350–356.
Grotenhuis, J., Smith, M., Lammeren, A., Stams, A. and Zehnder, A. (1991). Appl. Microb. Biotech., 36, 115–119.
Grotenhuis, J., Plugge, C.M., Stams, A. and Zehnder, A. (1992). Appl. Microb. Biotech., 58, 1054–1056.
Harada, H., Endo G., Tohya, Y. and Momonoi K. (1989). In: Proceedings of the 5th Int. Symposium on Anaerobic Digestion, A. Tilche and A. Rozzi (Eds.), pp. 1011–1020, Monduzzi Editore, Bologna, Italy.
Hulshoff Pol, L.W., Zeew, W., Velzeboer, C.T. and Lettinga, G. (1983). Wat. Sci. Tech., 15,8, 291–304.
Lettinga, G. and Hulshoff Pol, L.W. (1992). Wat. Sci. Tech., 24,8, 87–108.
Mendez-Pampin, R.J., Sierra Alvarez, R. and Hulshoff Pol, L.W. (1986). In: Anaerobic Treatment — a Grown-up Technology, NVA — EWPCA (Eds.), pp. 698–702. Industrial Presentations BV, Schiedam, The Netherlands.
Rouxhet, P.G. and Mozes, N. (1990). Wat. Sci. Tech., 22,1/2, 1–16.
Schmidt, J.E. and Ahring, B.K. (1996). Biotech. Bioeng., 49, 229–246.
Shen, C.D., Kosaric, N. and Blaszczyk, R. (1993). Water Res., 27,1, 25–33.
Yang, G. and Anderson, G.K. (1993). J. Environ. Eng., 119,5, 958–977.
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Brito, A., Rodrigues, A. & Melo, L. Granulation during the start-up of a UASB reactor used in the treatment of low strength wastewaters. Biotechnology Letters 19, 363–368 (1997). https://doi.org/10.1023/A:1018359102949
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DOI: https://doi.org/10.1023/A:1018359102949