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In vitro and in vivo studies to assess the effectiveness of cholestyramine as a binding agent for fumonisins

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Abstract

Several adsorbent materials were tested at 1 mg/ml for their in vitrocapacity to adsorb fumonisin B1 (FB1) from aqueous solutions. Cholestyramine showed the best adsorption capacity (85% from a solution containing 200 μg/ml FB1) followed by activated carbon (62% FB1). Bentonite adsorbed only 12% of the toxin from a solution containing 13 μg/ml FB1, while celite was not effective even at the lowest tested FB1 concentration (3.2 μg/ml). Cholestyramine was tested in vivoto evaluate its capacity to reduce the bioavailability of fumonisins (FBs) in rats fed diet contaminated with toxigenic Fusarium verticillioidesculture material. Rats were exposed for one week to FBs-free diet, FBs-contaminated diet containing 6 or 20 μg/g FB1 + FB2 and the same FBs-contaminated diet added of 20 mg/g cholestyramine. The increase of sphinganine/sphingosine (SA/SO) ratio in urine and kidney of treated rats was used as specific and sensitive biomarker of fumonisin exposure.The addition of cholestyramine to the FBs-contaminated diets consistently reduced the effect of FBs by reducing significantly (P < 0.05) both urinary and renal SA/SO ratios.

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Authors and Affiliations

  1. Istituto Tossine e Micotossine da Parassiti Vegetali, CNR, 70125, Bari, Italy

    Michele Solfrizzo, Angelo Visconti & Giuseppina Avantaggiato

  2. Department of Microbiology and Immunology, National University of Rio Cuarto, Rio Cuarto, Argentina

    Adriana Torres & Sofia Chulze

Authors
  1. Michele Solfrizzo
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  2. Angelo Visconti
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  3. Giuseppina Avantaggiato
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  4. Adriana Torres
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  5. Sofia Chulze
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Solfrizzo, M., Visconti, A., Avantaggiato, G. et al. In vitro and in vivo studies to assess the effectiveness of cholestyramine as a binding agent for fumonisins. Mycopathologia 151, 147–153 (2001). https://doi.org/10.1023/A:1017999013702

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