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Induction of Cyclosporin A-Sensitive Pore in Mitochondria of Intact Neurons during Uncoupling of Oxidative Phosphorylation

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Abstract

Using primary cultures of cerebellar granule cells we showed that Ca2+ transported into neurons under the effect of glutamate is accumulated and stored in mitochondria for a long time. Protonophore FCCP, an uncoupler of oxidative phosphorylation, stimulated the release of Ca2+ from mitochondria in a calcium-free medium in 81% glutamate-treated cells. Cyclosporin A and ATP-synthase blocker oligomycin decreased the number of cells with FCCP-induced Ca2+ release to 53 and 12%, respectively. Oligomycin partly prevented glutamate- and FCCP-induced decrease of intracellular ATP level.

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Authors and Affiliations

  1. Laboratory of Membranology, Center of Children Health, Russian Academy of Medical Sciences, Moscow

    T. P. Storozhevykh, A. I. Yuryavichyus, E. G. Sorokina & V. G. Pinelis

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  1. T. P. Storozhevykh
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  2. A. I. Yuryavichyus
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  3. E. G. Sorokina
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  4. V. G. Pinelis
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Storozhevykh, T.P., Yuryavichyus, A.I., Sorokina, E.G. et al. Induction of Cyclosporin A-Sensitive Pore in Mitochondria of Intact Neurons during Uncoupling of Oxidative Phosphorylation. Bulletin of Experimental Biology and Medicine 131, 440–443 (2001). https://doi.org/10.1023/A:1017963629229

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