Abstract
The mechanism of Zn resistance in multiple metal-resistant Pseudomonas putida strain S4 is based on inducible efflux. An ATPase in the strain S4 mediated active extrusion of Zn2+, which occurred during the exponential phase of growth. The ATPase activity was inhibited by micromolar concentrations (50 μM) of vanadate, suggesting the involvement of a P-type ATPase. The effluxed Zn2+ were not ejected out of the cell but stored in the outer membrane and periplasm, which provided the required binding sites. The strain S4, thus, employs a dual strategy of efflux and binding to bring about a proper management of essential ions like Zn.
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Choudhury, R., Srivastava, S. Mechanism of zinc resistance in Pseudomonas putida strain S4. World Journal of Microbiology and Biotechnology 17, 149–153 (2001). https://doi.org/10.1023/A:1016666000384
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DOI: https://doi.org/10.1023/A:1016666000384