Abstract
Purpose: Case studies of four in vitro fertilization (IVF) cycles where embryo transport by commercial airline followed by biopsy and genetic analysis with subsequent culture to Day 5 and resulting ongoing pregnancy.
Method: Retrospective clinical case study of 4 patients requiring preimplantation genetic diagnosis (PGD) testing. Normally fertilized embryos were transported in a battery-powered portable incubator by commercial airline following evaluation for fertilization under controlled conditions from the Center for Assisted Reproduction, Bedford, Texas to the Reproductive Genetic Institute, Chicago, Illinois. Following Day 3 embryo biopsy and genetic analysis, embryos were transported back to the Center for Assisted Reproduction for Day 5 embryo transfer.
Results: Ongoing clinical pregnancy resulted for all patients receiving embryo transfer.
Conclusion: These results demonstrate the feasibility of embryo transport by air for centers that do not have the in-house capabilities to perform genetic analysis. With successful pregnancies obtained through extended culture to Day 5, embryos requiring genetic analysis can be successfully transported by air, tested, and returned to the initial facility for embryo transfer without time restriction.
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REFERENCES
Alfonsin AE, Amato AR, Arrighi A, Blaquier JA, Cogorno M, Feldman ES, Echeverria FG, Horton M, Vecchia DLD, Millas N: Transport in vitro fertilization and intracytoplasmic sperm injection: Results of a collaborative trial. Fertil Steril 1998;69:66-70
Bucket WM, Fisch P, Dean NL, Biljan MM, Tan SL: In vitro fertilization and intracytoplasmic sperm injection pregnancies after successful transport of oocytes by airplane. Fertil Steril 1999;71:53-55
Coetsier T, Verhoeff A, De Sutter P, Roest J, Dhont M: Transport in vitro fertilization/intracellular sperm injection: A prospective randomized study. Hum Reprod 1997;12:54-56
Marek D, Langley M, Gardner DK, Confer N, Doody KM, Doody KJ: Introduction of blastocyst culture and transfer for all patients in an IVF program. Fertil Steril 1999;72:1035-1040
Gardner DK, Schoolcraft WB, Wagley L, Schlenker T, Stevens J, Hesla J: A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. Hum Reprod 1998;13:3434-3440
Van Steirteghem AC, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H, Derbe MP, Van Assche E, Devroey P: Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. A report of a second series of 300 consecutive treatment cycles. Hum Reprod 1992;8:1055-1060
Van Steirteghem AC, Nagy Z, Joris H, Liu J, Staessen L, Smitz J, Wisanto A, Devroey P: Higher fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993;8:1061-1066
Menezo Y, Nicollet B, Herbaut N, Andre D: Freezing cocultured human blastocysts. Fertil Steril 1992;58(5):977-980
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Langley, M., Marek, D., Cieslak, J. et al. Clinical Assisted Reproduction: Successful Day 5 Embryo Transfer and Pregnancies Resulting After Transport of Embryos by Air for Biopsy and Genetic Analysis. J Assist Reprod Genet 18, 330–335 (2001). https://doi.org/10.1023/A:1016624419635
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DOI: https://doi.org/10.1023/A:1016624419635