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Metabolite profiles of Stachybotrys isolates from water-damaged buildings and their induction of inflammatory mediators and cytotoxicity in macrophages

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Abstract

The metabolite profiles of 20 Stachybotrys spp.isolates from Finnish water-damaged buildings were compared with their biological activities. Effects of purified compounds on cytotoxicity and production of inflammatory mediators such as nitric oxide, IL-6 and TNFα in murine RAW264.7 macrophage cells were studied. The 11isolates belonging to the satratoxin-producing chemotype were highly cytotoxic to the macrophages. The isolates inducing inflammatory mediators all belonged to the atranone-producing chemotype, but pure atranones B, and D did not elicit a response in the bioassay. Altogether, cytotoxicity ofStachybotrys sp. isolates appear to be related to satratoxin production whereas the specific component inducing inflammatory responses in atranone-producing isolates remains obscure.

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Authors and Affiliations

  1. The Mycology Group, BioCentrum-DTU,Building 221, Technical University ofDenmark, DK-2800, Kgs. Lyngby, Denmark

    Kristian Fog Nielsen & Birgitte Andersen

  2. Energy and Indoor Climate Division, Danish BuildingResearch Institute, Dr. Neergaardsvej 15, DK-2970, Horsholm, Denmark

    Kristian Fog Nielsen

  3. Department of Environmental Health, National PublicHealth Institute, P.O. Box. 95, FIN-70701, Kuopio, Finland

    Kati Huttunen, Anne Hyvärinen & Maija-Riitta Hirvonen

  4. Department of Chemistry and Biochemistry and theJoint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD, 20742, USA

    Bruce B. Jarvis

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  1. Kristian Fog Nielsen
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  2. Kati Huttunen
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  4. Birgitte Andersen
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  6. Maija-Riitta Hirvonen
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Nielsen, K.F., Huttunen, K., Hyvärinen, A. et al. Metabolite profiles of Stachybotrys isolates from water-damaged buildings and their induction of inflammatory mediators and cytotoxicity in macrophages. Mycopathologia 154, 201–206 (2002). https://doi.org/10.1023/A:1016383402963

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