Abstract
To identify proteins associated with 5-fluorouracil-resistance, a comparative proteome analysis was carried out using human gastric cancer cells and their derivative cells made resistant to 5-fluorouracil. Five proteins, a heterogeneous nuclear ribonucleoprotein K, transcription factor II, FLJ11122, hook3 protein and T-complex locus TCP-1, were identified that were differentially expressed in these cell lines.
Similar content being viewed by others
References
Beck A, Etienne MC, Cheradame S, Fischel JL, Formento P, Renee N, Milano G (1994) A role for dihydropyrimidine dehydrogenase and thymidylate synthase in tumor sensitivity to fluorouracil. Eur. J. Cancer 30: 1517-1522.
Chang JW, Jeon HB, Lee JH, Yoo JS, Chun JS, Kim JH, Yoo YJ (2001) Augmented expression of peroxiredoxin I in lung cancer. Biochem. Biophys. Res. Comm. 289: 507-512.
Etienne MC, Cheradame S, Fischel JL, Formento P Dassonville O, Renee N, Schneider M, Thyss A, Demard F, Milano G (1995) Response to fluorouracil therapy in cancer patients: the role of tumoral dihydropyrimidine dehydrogenase activity. J. Clin. Oncol. 13: 1663-1670.
Kim CH, Kim KI, Hong SH, Lee YH, Chung IS (2001) Improved production of recombinant rotavirus VP6 in sodium butyratesupplemented suspension cultures of transgenic tomato cells. Biotechnol. Lett. 23: 1061-1066.
Kubota H, Matsumoto S, Yokota S, Yanagi H, Yura T (1999) Transcriptional activation of mouse cytosolic chaperonin CCT subunit genes by heat shock factors HSF1 and HSF2. FEBS Lett. 461: 125-129.
Lenz HJ, Leichman CG, Danenber KD, Danenberg PV, Groshen S, Cohen H, Laine L, Crookes P, Silberman H, Baranda J, Garcia Y, Li J, Leichman L (1995) Thymidylate synthase mRNA level in adenocarcinoma of the stomach: a predictor for primary tumor response and overall survival. J. Clin. Oncol. 14: 176-182.
McLeod HL, Sludden J, Murray GI, Keenan RA, Davidson AI, Park K, Koruth M, Cassidy J (1998) Characterization of dihydropyrimidine dehydrogenase in human colorectal tumor. Br. J. Cancer 77: 461-465.
Oliver FJ, Collins MKL, Lopez-Rivas A (1997) Overexpression of a heterologous thymidine kinase delays apoptosis induced by factor deprivation and inhibitors of deoxynucleotide metabolism. J. Biol. Chem. 272: 10624-10630.
Pinedo HM, Peters GFJ (1986) Fluorouracil. Biochemistry and pharmacology. J. Clin. Oncol. 6: 1653-1664.
Walenta JH, Didier AJ, Liu X, Kramer H (2001) The golgiassociated hook3 protein is a member of microtubule binding proteins. J. Cell. Biol. 152: 923-934.
Wang W, Marsh S, Cassidy J, McLeod HL (2001) Pharmacogenomic dissection of resistance to thymidylate synthase inhibitors. Cancer Res. 61: 5505-5510.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Park, S., Lee, M., Kim, HY. et al. Proteome analysis of 5-fluorouracil-resistance-associated protein in human gastric cancer cells. Biotechnology Letters 24, 1141–1145 (2002). https://doi.org/10.1023/A:1016122716886
Issue Date:
DOI: https://doi.org/10.1023/A:1016122716886