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125I-labeling and purification of peptide hormones and bovine serum albumin

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Abstract

The iodination and separation of various diagnostically and/or experimentally important peptides including (Tyr1)-somatostatin-14, rat Tyr-a-calcitonin gene-related peptide (23-37), motilin and vasoactive intestinal peptide, furthermore bovine serum albumin are described. All species were iodinated by the iodogen method. The 125I-labeled peptide products were separated by reversed-phase HPLC, the specific activities of mono-iodinated forms are near identical with the theoretical value. The labeled bovine serum albumin was separated by Sephadex G-100 gel filtration.

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Authors and Affiliations

  1. Department of Pharmacology and Pharmacotherapy, Neuropharmacology Research Group of the Hungarian Academy of Sciences, P. O. Box 99, H-7643, Pécs, Hungary

    J. Németh, B. Jakab & Z. Szilvassy

  2. Department of Experimental Surgery, University of Pécs, P. O. Box 99, H-7643, Pécs, Hungary

    G. Oroszi & E. Rőth

  3. Department of Dermatology, University of Pécs, P. O. Box 99, H-7643, Pécs, Hungary

    M.i Magyarlak & B. Farkas

Authors
  1. J. Németh
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  2. G. Oroszi
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  3. B. Jakab
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  4. M.i Magyarlak
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  5. Z. Szilvassy
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  6. E. Rőth
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  7. B. Farkas
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Németh, J., Oroszi, G., Jakab, B. et al. 125I-labeling and purification of peptide hormones and bovine serum albumin. Journal of Radioanalytical and Nuclear Chemistry 251, 129–133 (2002). https://doi.org/10.1023/A:1015062731674

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  • DOI: https://doi.org/10.1023/A:1015062731674

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