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Simultaneous Flow Cytometric Analysis of Phagocytosis and Oxidative Burst Activity in Equine Leukocytes

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Abstract

This paper describes a method for simultaneously measuring phagocytosis and oxidative burst activity in equine peripheral blood leukocytes by flow cytometry. Opsonized propidium iodide-labelled Staphylococcus aureus (PI-Sa) was used to measure the uptake of bacteria by equine phacocytes and the oxidative burst activity by oxidation of dihydrorhodamine 123. The requirements to achieve optimal activity of phagocytosis and oxidative burst are described. The advantage of the simultaneous technique is that it provides both independent and comparative values for phagocytosis and the oxidative burst, for the detection of impaired mechanisms of microbial destruction. Furthermore, the technique allows evaluation of opsonization activity in this context.

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Authors and Affiliations

  1. The James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Hungerford Hill Road, Ithaca, New York, 14853, USA

    M.J.B.F. Flaminio

  2. Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA

    B.R. Rush & E.G. Davis

  3. Agriculture and Animal Health Division, Bayer Corporation, Merriam, Kansas, USA

    K. Hennessy

  4. Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA

    W. Shuman & M.J. Wilkerson

Authors
  1. M.J.B.F. Flaminio
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  2. B.R. Rush
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  3. E.G. Davis
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  4. K. Hennessy
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  5. W. Shuman
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  6. M.J. Wilkerson
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Flaminio, M., Rush, B., Davis, E. et al. Simultaneous Flow Cytometric Analysis of Phagocytosis and Oxidative Burst Activity in Equine Leukocytes. Vet Res Commun 26, 85–92 (2002). https://doi.org/10.1023/A:1014033016308

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  • DOI: https://doi.org/10.1023/A:1014033016308

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