Production of medium chain length polyhydroxyalkanoates by fed-batch culture of Pseudomonas oleovorans

Abstract

Pseudomonas oleovorans was cultivated to produce medium chain length polyhydroxyalkanoates (MCL-PHAs) from octanoic acid and ammonium nitrate as carbon and nitrogen source, respectively, by a pH-stat fed-batch culture technique. The octanoate in the culture broth was maintained below 4 g l⁻¹ by feeding the mixture of octanoic acid and ammonium nitrate when the culture pH rose above 7.1. The final cell concentrations of 63, 55 and 9.5 g l⁻¹, PHA contents of 62, 75 and 67% of dry cell wt, and productivities of 1, 0.63 and 0.16 g l⁻¹ h⁻¹ were obtained when the C/N ratios in the feed were 10, 20 and 100 g octanoic acid g⁻¹ ammonium nitrate, respectively.