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Regeneration of almond from immature seed cotyledons

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Abstract

Adventitious shoots were regenerated from immature cotyledons of four almond cultivars (`Ne Plus Ultra', `Nonpareil', `Carmel' and `Parkinson'). Open-pollinated fruit were collected from orchard-grown trees 100–115 days after full bloom. The proximal ends of the cotyledons were excised and the embryonic axes discarded. The effects of different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA) and the presence or absence of light for the first 7 days of culture were determined. Shoot regeneration rates were highest for cotyledons cultured for 8 weeks on Murashige and Skoog (MS) basal medium containing TDZ (10.0 μM), followed by 4 weeks on medium without plant growth regulators. Regeneration levels were further improved if cotyledons were maintained in darkness for the first 7 days. IBA (0.5 μM) significantly reduced the development of adventitious shoots. The frequency of cotyledons that developed adventitious shoots under the optimum tested conditions for `Ne Plus Ultra', `Nonpareil', `Carmel', and `Parkinson' were 80.0%, 73.3%, 100.0% and 86.7%, respectively.

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Authors and Affiliations

  1. Department of Horticulture, Viticulture and Oenology, Plant Research Centre, Adelaide University, PMB1, Glen Osmond, Waite Campus, 5064, South Australia, Australia

    Phillip J. Ainsley, Terry Bertozzi, Graham G. Collins & Margaret Sedgley

  2. U.S. Department of Agriculture, Agricultural Research Service, Fruit Laboratory, Beltsville, MD, 20705, USA

    Freddi A. Hammerschlag

Authors
  1. Phillip J. Ainsley
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  2. Freddi A. Hammerschlag
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  3. Terry Bertozzi
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  4. Graham G. Collins
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  5. Margaret Sedgley
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Correspondence to Margaret Sedgley.

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Ainsley, P.J., Hammerschlag, F.A., Bertozzi, T. et al. Regeneration of almond from immature seed cotyledons. Plant Cell, Tissue and Organ Culture 67, 221–226 (2001). https://doi.org/10.1023/A:1012700714085

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