Abstract
Thogoto virus is a tick-borne member of the family Orthomyxoviridae. Previously, based on the similarity in antigenic relationship by cross-neutralization test, all virus strains were concluded to have derived from the same origin. In this study, we obtained partial gene sequences of 4 genes (PB1-like protein, PA-like protein, glycoprotein, and nucleoprotein) of 8 Thogoto virus strains isolated in Africa, Asia, and Europe and studied the genetic variation and phylogeny. Unrooted phylogenetic trees created by both neighbor-joining and maximum likelihood methods based on nucleotide and amino acid sequences for 4 genes were mostly similar and revealed two lineages, Euro-Asian and African. Intra-lineage nucleotide sequence variation was greater in the Euro-Asian lineage than in the African lineage for all 4 genes. Furthermore, for the strains of Euro-Asian lineage, variations for two genes associated with RNA-dependent RNA polymerase activities were greater than those for glycoprotein or nucleoprotein gene, based on both nucleotide and amino acid sequence differences as well as on synonymous and nonsynonymous differences, indicating greater mutation rates for the polymerase activity genes in these strains.
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Pringle C.R., Archiv Virol 141, 2251–2256, 1996.
Moore D.L., Causey O.R., Carey D.E., Reddy S., Cooke A.R., Akinguagbe F.M., David-West T.S., and Kemp G.E., Ann Trop Med Parasitol 69, 49–64, 1975.
Calisher C.H., Karabatsos N., and Filipe A.R., Am J Trop Med Hyg 37, 670–673, 1987.
Ogen-Odoi A., Miller B.R., Happ C.M., Maupin G.O., and Burkot T.R., Am J Trop Med Hyg 60, 439–440, 1999.
Kuno G. J Virol Methods 72, 27–41, 1998.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G., Nucleic Acids Res 25, 4876–4882, 1997.
Kumar S., Tamura K., and Nei M., Molecular evolutionary genetic analysis version 1.01. University Park, The Pennsylvania State University, 1993.
D'Erchia A.M., Gissi C., Pesole G., Saccone C., and Arnason U. Nature 381, 597–600, 1996.
Takezaki N., and Gojobori T., Mol Biol Evol 16, 590–601, 1999.
Srihongse S., Albanese M., and Casals J., Am J Tropl Med Hyg 23, 1161–1164, 1974.
Gorman O.T., Bean W.J., and Webster R.G., Curr Topic Microbiol Immunol 176, 75–97, 1992.
Scholtissek C., Virus Genes 11, 209–215, 1996.
Fitch W.M., Mol Phyl Evol 5, 247–258, 1996.
Morse M.A., Marriott A.C., and Nuttall P.A. Virology 186, 640–646, 1992.
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Kuno, G., Chang, GJ., Tsuchiya, K.R. et al. Phylogeny of Thogoto Virus. Virus Genes 23, 211–214 (2001). https://doi.org/10.1023/A:1011873028144
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DOI: https://doi.org/10.1023/A:1011873028144