Skip to main content
SpringerLink
Log in

3′-Terminal Sequence analysis of the RNA Genome of the Indian Isolate of Cardamom Mosaic Virus: A New Member of Genus macluravirus of potyviridae

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Cardamom mosaic virus, a possible member of the family Potyviridae has been associated with the mosaic disease (Katte disease) of small cardamom in India. A virus isolated from the symptomatic cardamom leaves was positive in ELISA only with antiserum to the Guatemalan isolate of cardamom mosaic virus and not with a number of other potyviruses. The size of the viral RNA (8.5 kb) and the molecular weight of the coat protein (CP) (38 kDa) were determined. A 1.8-kb product containing the partial nuclear inclusion body (NIb) gene, the entire coat protein gene and the 3′ untranslated region (UTR) was amplified by reverse transcription (RT) and polymerase chain reaction (PCR), cloned and sequenced. The viral origin of the clone was confirmed by Northern hybridization with viral RNA. The experimentally determined N-terminal sequence of the CP matched with the deduced amino acid sequence of the CP gene. Sequence analysis of the clone suggests that the cardamom mosaic virus is a member of the Macluravirus genus of the family Potyviridae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brunt A.A., Crabtree K., Dallwitz M.J., Gibbs A.J., and Watson L., Viruses of Plants — Description and Lists from the VIDE Database. CAB International, Wallingford, UK, 1996, pp. 233-234.

    Google Scholar 

  2. Saravanakumar M., Panicker S., and Usha R., Indian J Virol 14, 59-63, 1998.

    Google Scholar 

  3. Saju K.A., Venugopal M.N., and Mathew M.J., XII Annual convention — Abstract. Indian Virological Society, p.15, 1997.

  4. Varma P.M. and Capoor S.P., Indian J Agric Sci 28, 97-107, 1958.

    Google Scholar 

  5. Varmudi V., Spice India: Directorate of Economics and Statistics. New Delhi, April 2000, pp. 13-15.

  6. Uppal B.N., Varma P.M., and Capoor S.P., Curr Sci 14, 208-209, 1945.

    Google Scholar 

  7. Rajan P., J Plantation Crops 9, 34-41, 1981.

    Google Scholar 

  8. Naidu R. and Venugopal M.N., J Plantation Crops 16, 267-271, 1986.

    Google Scholar 

  9. Saigopal D.V.R., Naidu R., and Thomas J., J Plantation Crops 20, 73-75, 1992.

    Google Scholar 

  10. Gonsalves D., Trujillo E., and Hoch H.C., Plant Dis 70, 65-69, 1986.

    Google Scholar 

  11. Martin R.R. and Bristow P.R., Phytopathology 78, 1636-1640, 1988.

    Google Scholar 

  12. Laemmli U.K., Nature 227, 680-685, 1970

    PubMed  Google Scholar 

  13. Mishra A., McKimm-Breschkin J.L., Xiao X.W., and Shukla D.D., Indian J Virol 13(1), 15-22, 1997.

    Google Scholar 

  14. Mowat W.P. and Dawson S., J Virol Methods 15, 233-247, 1987.

    PubMed  Google Scholar 

  15. Farrcll R.E. Jr, RNA Methodologies: A Laboratory Guide for Isolation and Characterization. Academic Press, San Diego, 1993.

    Google Scholar 

  16. Carninci P., Nishiyama Y., Westover A., Itoh M., Nagaoka S., Sasaki N., Okazaki Y., Muramatsu M., and Hayashizaki Y., Proc Natl Acad Sci USA 95, 520-524, 1998.

    PubMed  Google Scholar 

  17. Gibbs A. and Mackenzie A., J Virol Methods 63, 9-16, 1997.

    PubMed  Google Scholar 

  18. Thompson J.D., Higgins D.G., and Gibson T.J., Nucleic Acids Res 22, 4673-4680, 1994.

    PubMed  Google Scholar 

  19. Felsenstein J., PHYLIP-Phylogeny Interphase Package (version 3.5c) http://evolution.genetics.washington.edu/phylip.html

  20. Shukla D.D. and Ward C.W., Adv Virus Res 36, 273-314, 1989.

    PubMed  Google Scholar 

  21. Ravi K.S., Joseph J., Nagaraju N., Prasad K.S., Reddy H.R., and Savithri H.S., Plant Dis 81, 673-676, 1997.

    Google Scholar 

  22. Badge J., Robinson D.J., Brunt A.A., and Foster G.D., J Gen Virol 78, 253-257, 1997.

    PubMed  Google Scholar 

  23. Shukla D.D., Ward C.W., and Brunt A.A., The Potyviridae, CAB International, Wallingford, 1994.

    Google Scholar 

  24. Carrington J.C. and Dougherty W.G., Proc Natl. Acad Sci USA 85, 3391-3395, 1988.

    PubMed  Google Scholar 

  25. Argos, P., Nucleic Acids Res 16, 9909-9916, 1988.

    PubMed  Google Scholar 

  26. Jacquet C., Delecolle B., Raccah B., Lecoq H., Dunez J., and Ravelonandro M., J Gen Virol 79, 1509-1517, 1998.

    PubMed  Google Scholar 

  27. Harrison B.D. and Robinson D.J., Molecular variation in vectorborne plant viruses: epidemiological significance. Philos Trans R Soc Lond B 321, 447-462, 1988.

    Google Scholar 

  28. Hall J.S., Adams B., Parsons T.J., French R., Lane L.C., and Jensen S.G., Mol Phylogenet Evol 10(3), 323-332, 1998.

    PubMed  Google Scholar 

  29. Shukla D.D. and Ward C.W., J Gen Virol 69, 2703-2710, 1988.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625021, India

    Thomas Jacob

Authors
  1. Thomas Jacob
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Usha
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jacob, T., Usha, R. 3′-Terminal Sequence analysis of the RNA Genome of the Indian Isolate of Cardamom Mosaic Virus: A New Member of Genus macluravirus of potyviridae. Virus Genes 23, 81–88 (2001). https://doi.org/10.1023/A:1011191614839

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011191614839

Search

Navigation