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Effect of Ischemia–Reperfusion on Bile Canalicular F-Actin Microfilaments in Hepatocytes of Human Liver Allograft

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Abstract

We studied and quantified the effect of ischemia–reperfusion on hepatic F-actin on bile canalicular and basolateral membranes in human liver allografts by means of confocal laser scanning microscopy imaging. The phalloidin–FITC staining of F-actin was normal in liver hepatocytes before reperfusion but decreased significantly after reperfusion (by 25% of controls). These results indicate that hepatic F-actin alteration is produced during the reperfusion phase. This modification, probably induced by reactive oxygen species, could impair bile canalicular contraction and tight junction permeability and consequently bile secretion in the postoperative period.

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Authors and Affiliations

  1. Laboratoire de Pathologie Cellulaire et Métabolique du Foie, INSERM U 559, Faculté de Médecine, 27 boulevard Jean Moulin, 13385, Marseille Cedex 05, France

    Liliane Benkoël, Frank Dodero & Dominique Lombardo

  2. Unité de Transplantation Hépatique, Hôpital de la Conception, 147 bd. Baille, 13385, Marseille Cedex 05, France

    Jean Hardwigsen, Pierre Campan, Yves Patrice Le Treut & Albert Chamlian

  3. Service d'Hépato-Gastroentérologie, Hôpital de la Conception, 147 bd. Baille, 13385, Marseille Cedex 05, France

    Danielle Botta-Fridlund

Authors
  1. Liliane Benkoël
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  2. Frank Dodero
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  3. Jean Hardwigsen
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  4. Pierre Campan
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  5. Danielle Botta-Fridlund
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  6. Dominique Lombardo
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  7. Yves Patrice Le Treut
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  8. Albert Chamlian
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Benkoël, L., Dodero, F., Hardwigsen, J. et al. Effect of Ischemia–Reperfusion on Bile Canalicular F-Actin Microfilaments in Hepatocytes of Human Liver Allograft. Dig Dis Sci 46, 1663–1667 (2001). https://doi.org/10.1023/A:1010693218680

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