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Transport and utilization of rhizoferrin bound iron in Mycobacterium smegmatis

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Abstract

Transport and metabolization of iron bound to the fungal siderophore rhizoferrin was analyzed by transport kinetics, Mössbauer and EPR spectroscopy. Saturation kinetics (v max=24.4 pmol/(mg min), K m=64.4μM) and energy dependence excluded diffusion and provided evidence for a rhizoferrin transport system in M. smegmatis. Based on the spectroscopic techniques indications for intracellular presence of the ferric rhizoferrin complex were found. This feature could be of practical importance in the search of novel drugs for the treatment of mycobacterial infections. EPR and Mössbauer spectroscopy revealed different ferritin mineral cores depending on the siderophore iron source. This finding was interpreted in terms of different protein shells, i.e. two types of ferritins.

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Authors and Affiliations

  1. Isotopenlabor, Technisch-Naturwissenschaftliche Fakultät, Medizinische Universität, D-23538, Lübeck, Germany

    Berthold F. Matzanke

  2. Mikrobiologie/Biotechnologie, Eberhard-Karls-Universität, D-72076, Tübingen, Germany

    Rudolf Böhnke & G. Winkelmann

  3. Hans-Knöll-Institut für Naturstoff-Forschung e.V. (HKI), D-07708, Jena, Germany

    Ute Möllmann & Gisbert Schumann

  4. Institut für Physik, Medizinische Universität, D-23538, Lübeck, Germany

    Volker Schünemann & Alfred X. Trautwein

Authors
  1. Berthold F. Matzanke
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  2. Rudolf Böhnke
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  3. Ute Möllmann
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  4. Volker Schünemann
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  5. Gisbert Schumann
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  6. Alfred X. Trautwein
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  7. G. Winkelmann
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Matzanke, B.F., Böhnke, R., Möllmann, U. et al. Transport and utilization of rhizoferrin bound iron in Mycobacterium smegmatis. Biometals 12, 315–321 (1999). https://doi.org/10.1023/A:1009274415607

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