Abstract
Human osteoblastic alveolar bone cells were cultured for 28 days in control conditions and in the presence of three non-lethal concentrations of AISI 316L stainless steel (SS) corrosion products. Cells were exposed to SS corrosion products in two experimental situations: (i) in selected stages of the incubation time (during the first, second, third and fourth week of culture); and (ii) during the 28 days incubation period. Cultures were characterized for cell proliferation, total protein content, alkaline phosphatase activity (ALP) and ability to form mineralized deposits; culture media was analyzed for ionized calcium (Ca) and phosphorus (P) concentrations throughout the incubation period. The presence of SS corrosion products during the different stages of the incubation period did not significantly affect the cell proliferation; however, a significant dose-dependent deleterious effect was observed on the levels and pattern of ALP activity, concentration of ionized Ca and P in the culture medium and, also, ability to form mineralized deposits, especially in cultures exposed during the first and second week of culture (respectively, lag phase and exponential cell growth phase). Similar effects were observed in cultures exposed to the SS corrosion products during the 28 days incubation period. However, the presence of such products during the third week (when the mineralization process occurs) and, also, during the fourth week, resulted in little or no significant effects on the behavior of alveolar bone cells. Results suggested that SS corrosion products above certain non-lethal concentrations may disturb the proliferation/differentiation relationship of osteoblastic human alveolar bone cell cultures. ©©2000 Kluwer Academic Publishers
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C. A. VAN BLITTERSWIJK, D. BAKKER, S. C. HESSELING and H. K. KOERTEN, Biomaterials 12 (1991) 187.
F. B. BAGAMBISA, H. F. KAPPERT and W. SCHILLI, J. Oral Maxillofac. Surg. 52 (1994) 52.
J. D. DE BRUIJN, C. A. VAN BLITTERSWIJK and J. E. DAVIES, J. Biomed. Mater. Res. 29 (1995) 89.
C. J. KIRKPATRICK, M. WAGNER, H. KOHLER, F. BITTINGER, M. OTTO AND C. L. KLEIN, J. Mater. Sci.: Mater. Med. 8 (1997) 131.
R. M. PILLIAR, J. Biomed. Mater. Res. 21 (1987) 1.
J. D. BOBYN, M. TANZER, J. J. DRYGIER, A. R. DU JOUNE and C. E. BROOKS, Clin. Orthop. 298 (1994) 27.
K. MERRITT and S. A. BROWN, in “Corrosion and Degradation of Implant Materials”, second symposium, ASTM STP 859, edited by A. C. Fraker and C. D. Griffin (American Society for Testing and Materials, Philadelphia, 1985) p. 195.
J. O. GALANTE, J. LEMONS, M. SPECTOR, P. D. WILSON and T. M. WRIGHT, J. Orthop. Res. 9 (1991) 760.
A. BARTOLOZZI and J. BLACK, Biomaterials 6 (1985) 2.
L. D. DORR, R. BLOCBAUM, J. EMMANUAL and R. MELDRUM, Clin. Orthop. 261 (1990) 82.
E. M. EVANS, M. A. R. FREEMAN, A. J. MILLER and B. VERNON-ROBERTS, J. Bone Joint Surg. 56B (1974) 626.
R. DEUTMAN, T. J. RULDER, R. BRIAN and J. P. NATER, ibid. 59A (1977) 862.
M. H. HUO, E. A. SALVATI, J. R. LIEBERMAN, F. BETTS and M. BANSAL, Clin. Orthop. 276 (1992) 157.
M. L. PEREIRA, A. M. ABREU, J. P. SOUSA and G. S. CARVALHO, J. Mater. Sci.: Mater. Med. 6 (1995) 523.
R. B. TRACANA, A. M. ABREU, J. P. SOUSA and G. S. CARVALHO, ibid. 6 (1995) 56.
J. Y. WANG, B. H. WICKLUND, R. B. GUSTILO and D. T. TSUKAYAMA, Biomaterials 17 (1996) 2233.
G. K. SMITH and J. BLACK, in “Corrosion and Degradation of Implant Materials”, second symposium, ASTM STP 859, edited by A. C. Fraker and C. D. Griffin (American Society for Testing and Materials, Philadelphia, 1985) p. 223.
I. BRAVO, S. R. SOUSA, G. S. CARVALHO and M. BARBOSA, in ``Advances in biomaterials”, edited by P. J. Doherty (Elsevier, Amsterdam, 1992) p. 7.
G. S. CARVALHO, M. CASTANHEIRA and I. DIOGO, J. Mater. Sci.: Mater. Med. 7 (1995) 77.
D. A. PULEO and W. W. HUH, J. Appl. Biomater. 6 (1995) 109.
G. J. THOMPSON and D. A. PULEO, ibid. 6 (1995) 249.
H. TOMÂS, G. S. CARVALHO, M. H. FERNANDES, A. P. FREIRE and L. M. ABRANTES, J. Mater. Sci.: Mater. Med. 7 (1996) 291.
Idem., ibid. 8 (1997) 233.
Z. L. SUN, J. C. WATAHA and C. T. HANKS, J. Biomed. Mater. Res. 34 (1997) 29.
S. MORAIS, J. P. SOUSA, M. H. FERNANDES and G. S. CARVALHO, Biomaterials 19 (1998) 13.
S. MORAIS, J. P. SOUSA, M. H. FERNANDES, G. S. CARVALHO, J. D. DE BRUIJN and C. A. VAN BLITTERSWIJK, ibid. 19 (1998) 999
Idem., J. Biomed. Mater. Res. (1998), 42 (1998) 199
M. H. FERNANDES, M. A. COSTA and G. S. CARVALHO, Pharmacol. Res. 31 (1995) 88.
Idem., J. Mater. Sci.: Mater. Med. 8 (1997) 61.
O. E. M. POHLER, in ``Biomaterials in reconstructive surgery”, edited by L. R. Rubin (C. V. Mosby, St. Louis, 1983) p. 158.
M. MCGEE-RUSSELL, J. Histochem. Cytochem. 6 (1958) 22.
C. MANIATOPOULOS, J. SODEK and A. MELCHER, Cell Tissue Res. 254 (1988) 317.
M. A. ARONOW, L. C. GERSTENFELD and T. A. OWEN, J. Cell Phys. 143 (1990) 213.
R. GUNDLE, C. J. JOYNER and J. T. TRIFFIT, Bone 16 (1995) 597.
T. A. OWEN, M. ARONOW and V. SHALHOUB, J. Cell Phys. 143 (1990) 420.
T. J. MARTIN, D. M. FINDLAY, J. K. HEATHK. and W. NG, in ``Handbook of experimental pharmacology”, edited by J. R. Mundy and T. J. Martin (Springer-Verlag, Berlin, 1993) p. 149.
G. S. STEIN and J. B. LIAN, in ``Cellular and molecular biology of bone”, edited by M. Noda (Academic Press Inc., Tokyo, 1993), p. 47.
C. G. BELLOWS, J. E. AUBIN and J. N. M. HEERSCHE, Bone and Min. 14 (1991) 27.
C. G. BELLOWS, J. N. M. HEERSCHE and J. E. AUBIN, ibid. 17 (1992) 15.
C. H. CHUNG, E. E. GOLUB and E. FORBES, Calcif. Tissue Int. 51 (1992) 305.
H. C. ANDERSON and D. C. MORRIS, in ``Handbook of experimental pharmacology”, edited by J. R. Mundy and T. J. Martin (Springer-Verlag, Berlin, 1993) p. 267.
G. S. STEIN and J. B. LIAN, Endocrinol. Rev. 4 (1995) 290.
T. RAE, J. Bone Joint Surg. 63-B (1981) 435.
M. WEBB and S. M. WEINZIERL, Brit. J. Cancer 26 (1972) 292.
S. A. KATZ and H. SALEM, J. Appl. Toxicol. 13 (1993) 217.
K. MERRITT, R. S. WORTMAN, M. MILLARD and S. A. BROWN, Biomater Med. Dev. Artif. Organs 11 (1983) 115.
L. C. LUCAS, L. J. BEARDEN and J. E. LEMONS, in “Corrosion and Degradation of Implant Materials”, second symposium, ASTM STP 859, edited by A. C. Fraker and C. D. Griffin (American Society for Testing and Materials, Philadelphia, 1985) p. 208.
V. BIANCHI, R. D. TOSO and P. DEBETTO, Toxicology 17 (1980) 219.
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Costa, M.A., Fernandes, M.H. Proliferation/differentiation of osteoblastic human alveolar bone cell cultures in the presence of stainless steel corrosion products. Journal of Materials Science: Materials in Medicine 11, 141–153 (2000). https://doi.org/10.1023/A:1008975507654
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DOI: https://doi.org/10.1023/A:1008975507654