Abstract
Lipase (EC 3.1.1.3) from Candida rugosa was covalently immobilised on to cellulose, cellulose derivatives (cellulose acetate and cellulose phthalate) and cellulose composite membranes using activating agents such as sodium periodate or carbodiimide. Other non-cellulosic polymeric membranes (nylon, polyurethane, chitosan and hydroxyethyl methacrylate-co-methyl methacrylate) were also prepared and used for lipase immobilisation. The results obtained showed that the expressed activities are of the same order of magnitude for similar enzyme loadings when compared with those obtained from literature.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bouwer ST, Cuperus FP, Derksen JTP (1997) The performance of enzyme-membrane reactors with immobilized lipase. Enzyme Microbiol. Technol. 21: 291-296.
Dharmsthiti S, Luchai S (1998) Production and immobilizationof lipase from Aeromonas sobria harboring a heterologous gene. J. Ferment. Bioeng. 86: 335-337.
Gemeiner P, Stefuca V, Bales V (1993) Biochemical engineering of biocatalysts immobilised on cellulosic materials. Enzyme Microbiol. Technol. 15: 551-566.
Gil MH, Piedade AP, Alegret S, Alonso J, Martinez-Fàbregas E, Orellana A (1992) Covalent binding of urease on ammonium-selective potentiometric membrane. Biosensors Bioelectronics 7: 645-652.
Guit RPM, Kloosterman M, Meindersma GW, Mayer M, Meijer EM (1991) Lipase kinetics: hydrolysis of triacetin by lipase from Candida cylindracea in a hollow-fiber membrane reactor. Biotechnol. Bioeng. 38: 727-732.
Gunnlaugsdottir H, Wannerberger K, Sivik B (1998), Alcoholysis and glyceride synthesis with immobilized lipase on controlledpore glass of varying hydrophobicity in supercritical carbon dioxide. Enzyme Microbiol. Technol. 22: 360-367.
Hoq MM, Yamane T, Shimizu S, Funada T, Ishida S (1985) Continuous hydrolysis of olive oil by lipase in microporous hydrophobic membrane bioreactor. J. Am. Oil Chem. Soc. 62: 1016-1021.
Murray M, Rooney D, Van Neikerk M, Montenegro A, Weatherley LR (1997) Immobilization of lipase onto lipophilic polymer particles and application to oil hydrolysis. Process Biochem. 32: 479-486.
Peled N, Krenz MC (1981) A new assay of microbial lipases with emulsified trioleoyl glycerol. Anal. Biochem. 112: 219-222.
Piedade AP, Guthrie JT, Kazlanciunas A, Gil MH (1995) Characterisation of cellulose derivatives — relevance to sensor development. Cellulose 2: 243-255.
Pronk W, Kerkhof PJAM, van Helden C, van 't Riet K (1988) The hydrolysis of triglycerides by immobilized lipase in a hydrophilic membrane reactor. Biotechnol. Bioeng. 32: 512-518.
Rucka M, Turkiewicz B (1990) Ultrafiltration membranes as carriers for lipase immobilization. Enzyme Microbiol. Technol. 12: 52-55.
Rucka M, Turkiewicz B, Zuk J (1990) Polymeric membranes for lipase immobilisation. J. Am. Oil Chem. Soc. 67: 887-889.
Shaw J-F, Chang RC, Wang FF, Wang YJ (1990) Lipolytic activities of a lipase immobilized on six selected supporting materials. Biotechnol. Bioeng. 35: 132-137.
Taylor F, Panzer CC, Craig JC Jr, O'Brien DJ (1986) Continuous hydrolysis of tallow with immobilized lipase in a microporous membrane. Biotechnol. Bioeng. 28: 1318-1322.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Carneiro-da-Cunha, M.G., Rocha, J.M.S., Garcia, F.A.P. et al. Lipase immobilisation on to polymeric membranes. Biotechnology Techniques 13, 403–409 (1999). https://doi.org/10.1023/A:1008913824571
Issue Date:
DOI: https://doi.org/10.1023/A:1008913824571