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Rapid method for trehalulose production and its purification by preparative high-performance liquid chromatography

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Biotechnology Techniques

Abstract

Trehalulose was produced with a good yield by enzymatic conversion of sucrose and easily purified by preparative HPLC using a single Ca2+-based column. In addition, the structure of this sugar was confirmed by 13C and 1H n.m.r studies.

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Authors and Affiliations

  1. Eridania-Béghin-Say, Institut National des Sciences Appliquées, Hall Gilbert Durand, Avenue de Rangueil, 31077, Toulouse Cedex, France

    Thierry Véronèse & Patrice Perlot

  2. Eridania-Béghin-Say, CEI, BP 2132, 27, boulevard du 11 novembre, 69603, Villeurbanne Cedex, France

    Alain Bouchu

Authors
  1. Thierry Véronèse
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  2. Alain Bouchu
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  3. Patrice Perlot
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Véronèse, T., Bouchu, A. & Perlot, P. Rapid method for trehalulose production and its purification by preparative high-performance liquid chromatography. Biotechnology Techniques 13, 43–48 (1999). https://doi.org/10.1023/A:1008857613103

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