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Engineering a CD4 mimetic inhibiting the binding of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein gp120 to human lymphocyte CD4 by the transfer of a CD4 functional site to a small natural scaffold

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Abstract

The solvent-exposed CDR2-like region of human CD4 was transferred to the structural scaffold of scorpion charybdotoxin, as a means to reproduce that site in a native-like conformation. The chimeric mini-protein (33 amino acids long), obtained by solid-phase synthesis, is able to specifically prevent the interaction of HIV-1 gp120 with CD4. This CD4 mimetic may represent a valuable tool in the study of the HIV–cell interactions and as a lead in the development of antiviral drugs.

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References

  1. Sweet, R.W., Truneh, A. and Hendrickson, W.A., Curr. Opin. Struct. Biol., 2 (1991) 622.

    Google Scholar 

  2. Capon, D.J. and Ward, R.H.R., Annu. Rev. Immunol., 9 (1991) 649.

    PubMed  Google Scholar 

  3. Fauci, A.S., Science, 384 (1996) 529.

    Google Scholar 

  4. Vita, C., Roumestand, C., Toma, F. and Ménez, A., Proc. Natl. Acad. Sci. USA, 92 (1995) 6404.

    PubMed  Google Scholar 

  5. Drakopoulou, E., Zinn-Justin, S., Guenneugues, M., Gilquin, B., Ménez, A. and Vita, C., J. Biol. Chem., 271 (1996) 11979.

    PubMed  Google Scholar 

  6. King, D.S., Fields, C.G. and Fields, G.B., Int. J. Pept. Protein Res., 36 (1990) 255.

    PubMed  Google Scholar 

  7. Bontems, F., Roumestand, C., Boyot, P., Gilquin, B., Doljanski, Y., Ménez, A. and Toma, F., Eur. J. Biochem., 196 (1991) 19.

    PubMed  Google Scholar 

  8. Bontems, F., Roumestand, C., Gilquin, B., Ménez, A. and Toma, F., Science, 254 (1991) 1521.

    PubMed  Google Scholar 

  9. Vita, C., Bontems, F., Bouet, F., Tauc, M., Poujeol, P., Vatanpour, H., Harvey, A.L., Ménez, A. and Toma, F., Eur. J. Biochem., 217 (1993) 157.

    PubMed  Google Scholar 

  10. Pierret, B., Virelizier, H. and Vita, C., Int. J. Pept. Protein Res., 46 (1995) 471.

    PubMed  Google Scholar 

  11. Yeh, P., Landais, D., Lemaitre, M., Maury, I., Crenne, J.-Y., Becquart, J., Murry-Brelier, A., Boucher, F., Montay, G., Fleer, R., Hirel, P.-H., Mayaux, J.-F. and Klatzmann, D., Proc. Natl. Acad. Sci. USA, 89 (1992) 1904.

    PubMed  Google Scholar 

  12. Clackson, T. and Wells, J.A., Science, 267 (1995) 383.

    PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. CEA, Département d'Ingénierie et d'Etudes des Protéines, C.E. Saclay, F-91190, Gif-sur-Yvette, France

    Eugenia Drakopoulou, Jean Vizzavona & Claudio Vita

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  1. Eugenia Drakopoulou
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  2. Jean Vizzavona
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  3. Claudio Vita
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Drakopoulou, E., Vizzavona, J. & Vita, C. Engineering a CD4 mimetic inhibiting the binding of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein gp120 to human lymphocyte CD4 by the transfer of a CD4 functional site to a small natural scaffold. Letters in Peptide Science 5, 241–245 (1998). https://doi.org/10.1023/A:1008837427367

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  • DOI: https://doi.org/10.1023/A:1008837427367

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