Abstract
In 1997 TTV was detected using representational difference analysis (RDA) in serum of a patient with posttransfusion hepatitis unrelated to known hepatitis viruses. The genome of TTV is a circular single-stranded DNA molecule of 3852 nt with negative polarity. TTV possibly can be grouped either into the existing family Circoviridae or into a recently established virus family “Circinoviridae”. Analysis of the complete DNA nucleotide sequence of TTV identified three partially overlapping open reading frames (ORFs). Neither DNA nucleotide nor corresponding amino acid sequences of TTV do show significant homologies to known sequences. TTV DNA nucleotide sequences amplified by PCR from sera of different patients show considerable sequence variations. Although the natural route of transmission of TTV is still unknown, there is clear evidence for a transmission of TTV through blood and blood products. TTV DNA can be detected in the feces of infected individuals suggesting that it may be possible to attract TTV infection from environmental sources. Since the discovery of TTV, numerous studies have investigated the prevalence of TTV infections in different human population groups all over the world. All these studies are based on PCR detection systems, but the technical aspects of the PCR systems vary significantly between the different investigators. The results of the epidemiological studies do not show a clear picture. The discovery of TTV as a viral agent and particularly the identification of a high percentage of infected carriers in the healthy human population raises the following questions: Firstly, what is the origin and molecular relatedness of TT virus. Secondly, what is the significance of TTV as a human pathogen. And thirdly, what are the exact molecular mechanisms of viral replication. To answer these questions it will be necessary to determine the primary structure and the coding capacity of several TTV patient isolates.
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Nishizawa T., Okamoto H., Konishi K., Yoshizawa H., Miyakawa Y., and Mayumi M., Biochem Biophys Res Commun 241, 92-97, 1997.
Okamoto H., Nishizawa T., Kato N., Ukita M., Ikeda H., Iizuka H., Miyakawa Y., and Mayumi M., Hepatol Res 10, 1-16, 1998.
Miyata H., Tsunoda H., Kazi A., Yamada A., Khan M.A., Murakami J., Kamahora T., Shiraki K., and Hino S., J Virol 73, 3582-3586, 1999.
Mushahwar I.K., Erker J.C., Muerhoff A.S., Leary T.P., Simons J.N., Birkenmeyer L.G., Chalmers M.L., Pilot-Matias T.J., and Dexai S.M., Proc Natl Acad Sci USA 96, 3177-3182, 1999.
Lukert P., de Boer G.F., Dale J.L., Keese P., McNulty M.S., Randles J.W., and Tischer I., in Murphy F.A., Fauquet C.M., Bishop D.H.L., Ghabrial S.A., Jarvis A.W., Martelli G.P., Mayo M.A., and Summers M.D. (eds). “Virus Taxonomy”. Sixth Report of the International Committee on Taxonomy of Viruses. Springer-Verlag, Vienna & New York, 166-168, 1995.
Claessens J.A., Schrier C.C., Mockett A.P., Jagt E.H., and Sondermeijer P.J., J Gen Virol 72, 2003-2006, 1991.
Bassami M.R., Berryman D., Wilcox G.E., and Raidal S.R., Virology 249, 453-459, 1998.
Meehan B.M., Creelan J.L., McNulty M.S., and Todd D., J Gen Virol 78, 221-227, 1997.
Niagro F.D., Forsthoefel A.N., Lawther R.P., Kamalanathan L., Ritchie B.W., Latimer K.S., and Lukert P.D., Arch Virol 143, 1723-1744, 1998.
Mankertz J., Buhk H.J., Blaess G., and Mankertz A., Virus Genes 16, 267-276, 1998.
Todd D., Creelan J.L., Meehan B.M., and McNulty M.S., Arch Virol 141, 1523-1534, 1996.
Phenix K.V., Meehan B.M., Todd D., and McNulty M.S., J Gen Virol 75, 905-909, 1994.
Todd D., Niagro F.D., Ritchie B.W., Curran W., Allan G.M., Lukert P.D., Latimer K.S., Steffens W.L., and McNulty M.S., Arch Virol 117, 129-135, 1991.
Takahashi K., Ohta Y., and Mishiro S., Hepatol Res 12, 111-120, 1998.
Biagini P., Gallian P., Attoui H., Cantaloube J.F., de Mícco P., and de L.X., J Gen Virol 80, 419-424, 1999.
Viazov S., Ross R.S., Niel C., de Oliveira J.M., Varenholz C., Da Villa G., and Roggendorf M., J Gen Virol 79, 3085-3089, 1998.
Tanaka Y., Mizokami M., Orito E., Ohno T., Nakano T., Kato T., Kato H., Mukaide M., Park Y.M., Kim B.S., and Ueda R., FEBS Lett 437, 201-206, 1998.
Sumazaki R., Yamada-Osaki M., Kajiwara Y., Shirahata A., and Matsui A., Lancet 352, 1308-1309, 1998.
Yamada-Osaki M., Sumazaki R., Noguchi E., Shibasaki M., and Matsui A., Lancet 352, 1309-1310, 1998.
Hirata D., Kaneko N., Iwamoto M., Yoshio T., Okazaki H., Mimori A., Masuyama J., and Minota S., Br J Rheumatol 37, 1361-1362, 1998.
Takahashi K., Hoshino H., Ohta Y., Yoshida N., and Mishiro S., Hepatol Res 12, 233-239, 1998.
Takayama S., Miura T., Matsuo S., Taki M., and Sugii S., Br J Haematol 104, 626-629, 1999.
Goto K., Sugiyama K., Terabe K., Mizutani F., and Wada Y., J Med Virol 57, 405-407, 1999.
Ikeda H., Takasu M., Inoue K., Okamoto H., Miyakawa Y., Mayumi M., Takayama S., Yamazaki S., Matsuo S., and Sugii S., J Hepatol 30, 205-212, 1999.
Oguchi T., Tanaka E., Orii K., Kobayashi M., Hora K., and Kiyosawa K., J Gastroenterol 34, 234-240, 1999.
Kanda Y., Tanaka Y., Kami M., Saito T., Asai T., Izutsu K., Yuji K., Ogawa S., Honda H., Mitani K., Chiba S., Yazaki Y., and Hirai H., Blood 93, 2485-2490, 1999.
Umemura T., Tanaka E., Ota M., Orii K., Yoshizawa H., Imai H., Sodeyama T., and Kiyosawa K., Hepatol Res 13, 212-220, 1999.
Fukuda Y., Nakano I., Katano Y., Kumada T., Hayashi K., Nakano S., and Hayakawa T., Infection 27, 125-127, 1999.
Tanaka H., Okamoto H., Luengrojanakul P., Chainuvati T., Tsuda F., Tanaka T., Miyakawa Y., and Mayumi M., J Med Virol 56, 234-238, 1998.
Poovorawan Y., Theamboonlers A., Jantaradsamee P., Kaew-in N., Hirsch P., and Tangkitvanich P., Infection 26, 355-358, 1998.
Tangkijvanich P., Hirsch P., Theamboonlers A., Nuchprayoon I., and Poovorawan Y., J Gastroenterol 34, 227-233, 1999.
Nakano T., Park Y.M., Mizokami M., Choi J.Y., Orito E., Ohno T., Kato T., Kondo Y., Tanaka Y., Kato H., and Kim B.S., J Hepatol 30, 389-393, 1999.
Prescott L.E. and Simmonds P., Lancet 339, 776-777, 1998.
Simmonds P., Davidson F., Lycett C., Prescott L.E., MacDonald D.M., Ellender J., Yap P.L., Ludlam C.A., Haydon G.H., Gillon J., and Jarvis L.M., Lancet 352, 191-195, 1998.
Naoumov N.V., Petrova E.P., Thomas M.G., and Williams R., Lancet 352, 195-197, 1998.
MacDonald D.M., Scott G.R., Clutterbuck D., and Simmonds P., J Infect Dis 179, 686-689, 1999.
Höhne M., Berg T., Müller A.R., and Schreier E., J Gen Virol 79, 2761-2764, 1998.
Viazov S., Ross R.S., Varenholz C., Lange R., Holtmann M., Niel C., and Roggendorf M., J Clin Virol 11, 183-187, 1998.
Schröter M., Feucht H., Zöllner B., Knödler B., Fischer L., and Laufs R., Hepatol Res 13, 205-211, 1999.
Gerolami V., Halfon P., Chambost H., Thuret I., and Halimi G., Lancet 352, 1309-1309, 1998.
Halfon P., Bourliere M., Feryn J.M., Khiri H., Chanas M., Salvadori J.M., and Ouzan D., J Hepatol 30, 552, 1999.
Prati D., Lin Y.H., De Mattei C., Liu J.K., Farma E., Ramaswamy L., Zanella A., Lee H., Rebulla P., Allain J.P., Sirchia G., and Chen B., Blood 93, 1502-1505, 1999.
Toniutto P., Fabris C., Falleti E., Lombardelli T., Gasparini V., Barillari G., Biffoni F., and Pirisi M., Blood 2426-2427, 1999.
Charlton M., Adjei P., Poterucha J., Zein N., Moore B., Therneau T., Krom R., and Wiesner R., Hepatology 28, 839-842, 1998.
Desai S.M., Muerhoff A.S., Leary T.P., Erker J.C., Simons J.N., Chalmers M.L., Birkenmeyer L.G., Pilot-Matias T.J., and Mushahwar I.K., J Infect Dis 179, 1242-1244, 1999.
Niel C., de Oliveira J.M., Ross R.S., Gomes S.A., Roggendorf M., and Viazov S., J Med Virol 57, 259-263, 1999.
Tanaka Y., Mizokami M., Orito E., Nakano T., Kato T., Ding X., Ohno T., Ueda R., Sonoda S., Tajima K., Miura T., and Hayami M., J Med Virol 57, 264-268, 1999.
Davidson F., MacDonald D., Mokili J.L., Prescott L.E., Graham S., and Simmonds P., J Infect Dis 179, 1070-1076, 1999.
Takayama S., Yamazaki S., Matsuo S., and Sugii S., Biochem Biophys Res Commun 256, 208-211, 1999.
Simmonds P., Davidson F., and Jarvis L.M., Lancet 352, 1310-1311, 1999.
Tsuda F., Okamoto H., Ukita M., Tanaka T., Akahane Y., Konishi K., Yoshizawa H., Miyakawa Y., and Mayumi M., J Virol Methods 77, 199-206, 1999.
Okamoto H., Kato N., Iizuka H., Tsuda F., Miyakawa Y., and Mayumi M., J Med Virol 57, 252-258, 1999.
Okamura A., Yoshioka M., Kubota M., Kikuta H., Ishiko H., and Kobayashi K., J Med Virol 58, 174-177, 1999.
Fujiwara T., Iwata A., Iizuka H., Tanaka T., and Okamoto H., Lancet 352, 1310-1310, 1999.
Kobayashi M., Chayama K., Arase Y., Tsubota A., Suzuki Y., Koida I., Saitoh S., Murashima N., Ikeda K., Koike H., Hashimoto M., and Kumada H., J Gastroenterol Hepatol 14, 358-363, 1999.
Yokozaki S., Fukuda Y., Nakano I., Katano J., Okamuru M., and Naruse M., Blood 93, 3569-3570, 1999.
Okamoto H., Akahane Y., Ukita M., Fukuda M., Tsuda F., Miyakawa Y., and Mayumi M., J Med Virol 56, 128-132, 1998.
Cossart Y., Lancet 352, 164, 1998.
Imawari M., J Gastroenterol 34, 292-293, 1999.
Zuckerman A.J., Lancet 353, 932, 1999.
Erker J.C., Leary T.P., Desai S.M., Chalmers M.L., and Mushahwar I.K., J Gen Virol 80, 1743-1750, 1999.
Hijikata M., Takahashi K., and Mishiro S., Virology 260, 17-22, 1999.
Okamoto H., Nishizawa T., Ukita M., Takahashi M., Fukuda M., Iizuka H., Miyakawa Y., and Mayumi M., Virology 259, 437-448, 1999.
Okamoto H., Takahashi M., Nishizawa T., Ukita M., Fukuda M., Tsuda F., Miyakawa Y., and Mayumi M., Virology 259, 428-436, 1999.
Leary T.P., Erker J.C., Chalmers M.L., Desai S.M., and Mushahwar I.K., J Gen Virol 80, 2115-2120, 1999.
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Springfeld, C., Bugert, J.J., Schnitzler, P. et al. TT Virus as a Human Pathogen: Significance and Problems. Virus Genes 20, 35–45 (2000). https://doi.org/10.1023/A:1008156022845
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DOI: https://doi.org/10.1023/A:1008156022845