Bacteriophage λ cIII Gene Product has an Additional Function Apart from Inhibition of cII Degradation

Abstract

For lysogenization of Escherichia coli cells by bacteriophage λ, functions of three λ genes called c are necessary. The cI gene codes for a repressor that blocks activities of lytic promoters. However, early after infection, expression of cI is dependent on the function of the cII gene, coding for a specific transcriptional activator. The cII protein is unstable in E. coli cells due to FtsH-mediated proteolysis. The cIII gene product is an inhibitor of the FtsH protease. Here we demonstrate that cIII may have another function apart from inhibition of cII degradation. We found that overexpression of the cII gene results in impaired lysogenization by phage λ, however simultaneous overexpression of the cIII gene abolished this negative effect on lysogenization. Analysis of cII-mediated transcriptional activation of certain promoters at different levels of cII and cIII proteins in cells confirmed that observed effects cannot be explained assuming that the only role of cIII is inhibition of FtsH-mediated degradation of cII. We propose that cIII has an additional role apart from its well-known function in indirect stabilization of cII. Apparently, cIII influences not only cII level but also activity of this transcriptional stimulator, especially at its high concentrations.