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Complete Nucleotide Sequence and Infectious cDNA Clone of the RNA1 of a Chinese Isolate of Broad Bean Wilt Virus 2

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Abstract

The nucleotide sequence of the RNA1 of broad bean wilt virus 2 (BBWV2) isolate B935 has been determined from overlapping cDNA clones. It contains 5956 nucleotides in length excluding the 3′ terminal poly(A) tail and contains a single long open reading frame (ORF) of 5613 nucleotides extending from nucleotide 234 to 5846. A repeated motif has been found in the 5′ non-coding region. The predicted polyprotein encoded by the long ORF is 1870 amino acid in length with a molecular weight of 210 K. Amino acid sequence comparisons between portions of the BBWV2 RNA1-encoded polyprotein and proteins encoded by several species in Comoviridae revealed the putative functions of BBWV2 RNA1-encoded proteins and the same general genetic organization as that of comoviruses and nepoviruses. Based on the determined sequence, full-length cDNA clone of RNA1 designated as pU1FL was constructed. Together with transcripts from full-length cDNA clone of RNA2 (pU2FL), transcripts from pU1FL infected Chenopodium quinoa successfully.

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Authors and Affiliations

  1. Institute of Biotechnology, Zhejiang University, Huajiachi, Hangzhou, 310029, People's Republic of China

    Yijun Qi, Xueping Zhou & Debao Li

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  1. Yijun Qi
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  2. Xueping Zhou
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  3. Debao Li
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Qi, Y., Zhou, X. & Li, D. Complete Nucleotide Sequence and Infectious cDNA Clone of the RNA1 of a Chinese Isolate of Broad Bean Wilt Virus 2. Virus Genes 20, 201–207 (2000). https://doi.org/10.1023/A:1008132310111

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