Abstract
The nucleotide sequence of the coat protein gene of the dwarfing (D) strain of soybean dwarf luteovirus (SbDV) was determined from cloned cDNA. The gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated molecular mass of 22.2 kDa. A major portion of the coat protein open reading frame (ORF) was expressed in Escherichia coli as a pET fusion protein and the product was detected by western blot analysis using SbDV-D polyclonal antibodies. Comparison of the deduced coat protein amino acid sequence to that from the yellowing (Y) strain of SbDV demonstrated 88% identity.
Similar content being viewed by others
References
Murphy F.A., Fauquet C.M., Bishop D.H.L., Ghabriel S.A., Jarvis A.W., Martelli G.P., Mayo M.A., and Summers M.D., “Sixth Report of the International Committee on Taxonomy of Viruses”. Springer-Verlag, Wien, pp. 379-383, 1995.
Tamada T. and Kojima M., Descriptions of Plant Viruses. CMI/AAB, Kew, England, no. 179, 1979.
Tamada T., Ann Phytopathol Soc Jpn 39, 27-34, 1973.
Smith O.P., Hunst P.L., Hewings A.D., Stone A.L., Tolin S.A., and Damsteegt V.D., Phytopathology 81, 131-134, 1991.
Hewings A.D., Damsteegt V.D., Sindermann A.E., and Tolin S.A., Plant Dis 74, 844-848, 1990.
Damsteegt V.D. and Voegtlin D.J., Ann Entomol Soc Am 83, 949-955, 1990.
Smith O.P., Damsteegt V.D., Harris K.F., and Vonder Haar R., Arch Virol 133, 223-231, 1993.
Sambrook J., Fritisch E.F., and Maniatis T., Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York, 1989.
Feinberg A.P. and Vogelstein B., Anal Biochem 132, 6-13, 1983.
Giesecke H., Obermaier B., Domdey H., and Neubert W.J., J Virol Methods 38, 47-60, 1992.
Devereux J., Haeberli P., and Smithies O., Nucleic Acids Res 12, 387-395, 1984.
Studier F.W., Rosenberg A.H., Dunn J.J., and Dubendorff J.W., Meth Enzymol 185, 60-89, 1990.
Scharf S.J., in Innis M.A., Gelfand D.H., Sninsky J.J., and White T.J., (eds). “PCR Protocols: A Guide to Methods and Applications”, Academic Press, New York, pp. 84-91, 1990.
Harlow E. and Lane D., Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1988.
Laemmli U.K., Nature (London) 227, 680-685, 1970.
Miller W.A., Dinesh S.P., and Paul C.P., Crit Rev Plant Sci 14, 179-211, 1995.
Tacke E., Schmitz J., Prufer D., and Rhode W., Virology 197, 274-282, 1993.
Halling S.M. and Smith S., Bio/Technology 3, 715-720, 1985.
Branden C. and Tooze J., Introduction to Protein Structure. Garland Publishing, New York, 1991.
Nikolaeva O.V., Karasev A.V., Gumpf D.J., Lee R.F., and Garnsey S.M., Phytopathology 85, 691-694, 1995.
Hewings A.D., Damsteegt V.D., and Tolin S.A., Phytopathology 76, 759-763, 1986.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Smith, O.P., Durkin, S.A., Luster, D.G. et al. Sequence and Expression in Escherichia coli of the Coat Protein Gene of the Dwarfing Strain of Soybean Dwarf Luteovirus. Virus Genes 17, 207–211 (1998). https://doi.org/10.1023/A:1008012219123
Issue Date:
DOI: https://doi.org/10.1023/A:1008012219123