Abstract
Numerous investigators have developed monoclonal antibodies against B-cell alloantigen(s) of rheumatic fever. However, the developed monoclonals do not have the same significance in all the populations. We have developed a battery of monoclonals against B-cell alloantigens of North Indian rheumatic fever patients. In the present study, we have used these monoclonals to examine the frequency of rheumatic antigens in 30 patients with recurrence of rheumatic activity (RRA), 30 of rheumatic heart disease (RHD) patients and 50 controls using alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. These patients were examined at the time of registry and after three months follow up. RRA patients showed higher percentage of lymphocyte positive as compare to RHD and controls. Interestingly, On follow-up RRA patients showed significant decline in positive lymphocyte as compare to first visit whereas no such change was observed in RHD patients. There were 90–93% of RRA and RHD patients positive with these monoclonals. A significant age variation of rheumatic cells was also noticed in all groups of rheumatic patients. We conclude that monoclonals raised from the same ethnic population are highly specific and cost effective to use them to develop an easy field test system such as APAAP, to identify the individual at risk, to develop rheumatic fever. It is also suggested that the alloantigen marker may persist through out life and gets activated after recurrence of the disease.
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References
Kumar D, Al-Bader A, Dashti H, Abul H, Ganguly NK, Singal PK: Molecular concepts in rheumatic fever and rheumatic heart disease. Exp Clin Cardiol 2: 155–160, 1997
Padmavati S: Epidemiology of cardiovascular disease in India: Rheumatic heart disease. Circulation 25: 703–710, 1962
Grover A, Dhawan A, Iyengar SD, Anand IS, Wahi PL, Ganguly NK: Epidemiology of rheumatic fever and rheumatic heart disease in rural community in Northern India. Bull WHO 71: 59–66, 1993
Stollerman GH: Rheumatic fever. In: L.E. Holt, R. McIntosh, H.L. Banet (eds). Pediatrics, 13th edn. Appleton-Century Crafts, New York, 1962, p 781
Zabriskie JB, Iaveney D, Williams RC, Fu SM, Yeadon CA, Fotino M, Braun DG: Rheumatic fever associated B-cell alloantigen as identified by monoclonal antibodies. Arth Rheumatol 28: 1047–1051, 1985
Patarroyo ME, Winchestev RJ, Vejerano A, Gibofsky A, Chalem F, Zabriskie JB, Kunkel HG: Association of a B-cell alloantigen with susceptibility to rheumatic fever. Nature (Lond) 278: 173–174, 1979
Khanna, Ashwani K, Danial R, Buskirk William A, Gibofsky MK, Crow A, Menon M, Fortiro HM, Reid Theio Roinking R, Ribinstein P, Zabriskie JB: Presence of a non-HLA B cell antigen in rheumatic fever patients and their families as defined by a monoclonal antibody. J Clin Invest 83: 1710–1716, 1989
Ganguly NK, Anand IS, Koicha M, Jindal S, Wahi PL: Frequency of D8/17 B lymphocyte alloantigen in North Indian patients with rheumatic heart disease. Immunol Cell Biol 70: 9–14, 1992
Taneja V, Mehra NK, Reddy KS et al.: HLA-DR/DQ antigens and reactivity to B-cell alloantigen D8/17 in Indian patients with rheumatic heart disease. Circulation 80: 335–340, 1989
Kumar D, Kaur S, Grover A, Bali H, Khanduja KL, Kaplan EL, Gray ED, Ganguly NK: Further observations and characterization of monoclonal antibodies reacting with B-cell alloantigens associated with rheumatic fever and rheumatic heart disease. J Lab Clin Med 135: 287–293, 2000
Kaur S, Kumar D, Grover A, Khanduja KL, Kaplan EL, Gray ED, Ganguly NK: Ethnic differences in expression of susceptibility marker(s) in rheumatic fever/rheumatic heart disease patients. Int J Cardiol 64: 9–14, 1998
Danajani AS et al.: Guidelines for the diagnosis of rheumatic fever: Jones criteria, updated 1992. Circulation 87: 302–307, 1993
Erber WN, Pinching AJ, Mason DY: Immunocytochemical detection of T and B cell population in routine blood smears. Lancet 1: 1042-1046, 1984
Veasy IG, Wiedmeier SE, Orsmond GS, Ruttenberg HD, Boucek MM, Roth SJ, Tait VF, Thompson JA, Daly JA, Kaplan EL, Hill HR: Resurgence of acute rheumatic fever in the intermountain area of the United States. N Engl J Med 316: 421–427, 1987
Kumar D, Kaur S, Grover A, Singal PK, Ganguly NK: An easy method for detection of rheumatic antigen(s) in rheumatic fever/rheumatic heart disease patients by dot-Elisa. Can J Cardiol 14: 807–810, 1998
Regelmann WE, Talbot R, Cairns L, Matin D, Miller LC, Zabriskie JB, Braun D, Gray ED: Distribution of cells bearing ‘rheumatic’ antigens in peripheral blood of patients with rheumatic fever/rheumatic heart disease. J Rheumatol 16: 931–935, 1989
Gray ED, Regelmann WE, Abdin Z, Kholy A El, Zaher S, Kamel R, Mansour M, Miller L, Ferrieri P, Zabriskie JB, Braun D: Compartmentalization of cells bearing ‘rheumatic’ cell surface antigens in peripheral blood and tonsils in rheumatic heart disease. J Infect Dis 155: 247–252, 1987
Regelmann WE, Gray ED, Wannamaker LW, Lebien TW, Mansour M, el Kholy A, Abdin Z: Lymphocyte subpopulations in rheumatic heart disease. J Rheumatol 14: 23–27, 1987
Vijaykumar M, Narula J, Reddy KS, Kaplan EL: Incidence of rheumatic fever and prevalence of rheumatic heart disease in India. Int J Cardiol 43: 221–228, 1994
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Kumar, D., Kaul, P., Grover, A. et al. Distribution of cells bearing B-cell alloantigen(s) in North Indian rheumatic fever/rheumatic heart disease patients. Mol Cell Biochem 218, 21–26 (2001). https://doi.org/10.1023/A:1007204208281
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DOI: https://doi.org/10.1023/A:1007204208281