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Effects of peroxide on contractility of coronary artery rings of different sizes

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Abstract

Reactive oxygen species (ROS, free radicals) produced during cardiac ischemia and reperfusion can damage the contractile functions of arteries. The sarcoplasmic reticulum (SR) Ca2+ pump in coronary artery smooth muscle is very sensitive to ROS. Here we show that contractions of de-endothelialized rings from porcine left coronary artery produced by the hormone Angiotensin II and by the SR Ca2+ pump inhibitors cyclopiazonic acid and thapsigargin correlate negatively with the tissue weight. In contrast, the contractions due to membrane depolarization by high KCl correlate positively. Peroxide also produces a small contraction which correlates negatively with the tissue weight. When artery rings are treated with peroxide and washed, their ability to contract with Angiotensin II, cyclopiazonic acid and thapsigargin decreases. Thus, the SR Ca2+ pump may play a more important role in the contractility of the smaller segments of the coronary artery than in the larger segments. These results are consistent with the hypothesis that ROS which damage the SR Ca2+ pump affect the contractile function of the distal segments more adversely than of the proximal segments.

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References

  1. Singal PK, Dhalla AK, Hill M, Thomas TP: Endogenous antioxidant changes in the myocardium in response to acute and chronic stress conditions. Mol Cell Biochem 129: 179–186, 1993

    PubMed  Google Scholar 

  2. Downey JM: Free radicals and their involvement during long-term myocardial ischemia and reperfusion. [Review]. Ann Rev Physiol 52: 487–504, 1990

    Google Scholar 

  3. Halliwell B, Hoult JR, Blake DR: Oxidants, inflammation, and anti-inflammatory drugs. [Review]. FASEB J 2: 2867–2873, 1988

    PubMed  Google Scholar 

  4. Kukreja RC, Okabe E, Schrier GM, Hess ML: Oxygen radical-mediated lipid peroxidation and inhibition of Ca2+-ATPase activity of cardiac sarcoplasmic reticulum. Arch Biochem Biophys 261: 447–457, 1988

    PubMed  Google Scholar 

  5. Vlessis AA, Muller P, Bartos D, Trunkey D: Mechanism of peroxideinduced cellular injury in cultured adult cardiac myocytes. FASEB J 5: 2600–2605, 1991

    PubMed  Google Scholar 

  6. Dhalla NS, Dixon IM, Rupp H, Barwinsky J: Experimental congestive heart failure due to myocardial infarction: Sarcolemmal receptors and cation transporters. Basic Res Cardiol 86(Suppl 3): 13–23, 1991

    Google Scholar 

  7. Burton KP, Morris AC, Massey KD, Buja LM, Hagler HK: Free radicals alter ionic calcium levels and membrane phospholipids in cultured rat ventricular myocytes. J Mol Cell Cardiol 22: 1035–1047, 1990

    PubMed  Google Scholar 

  8. Kaneko M, Masuda H, Suzuki H, Matsumoto Y, Kobayashi A, Yamazaki N: Modification of contractile proteins by oxygen free radicals in rat heart. Mol Cell Biochem 125: 163–169, 1993

    PubMed  Google Scholar 

  9. Okabe E, Kuse K, Sekishita T, Suyama N, Tanaka K, Ito H: The effect of ryanodine on oxygen free radical-induced dysfunction of cardiac sarcoplasmic reticulum. J Pharmacol Exp Therap 256: 868–875, 1991

    Google Scholar 

  10. Luscher TF, Tanner FC, Tschudi MR, Noll G: Endothelial dysfunction in coronary artery disease. [Review]. Ann Rev Med 44: 395–418, 1993

    PubMed  Google Scholar 

  11. Dreher D, Junod AF: Differential effects of superoxide, hydrogen peroxide, and hydroxyl radical on intracellular calcium in human endothelial cells. J Cell Physiol 162: 147–153, 1995

    PubMed  Google Scholar 

  12. Elliott SJ, Doan TN: Oxidant stress inhibits the store-dependent Ca(2+)-influx. pathway of vascular endothelial cells. Biochem J 292: 385–393, 1993

    PubMed  Google Scholar 

  13. Grover AK, Samson SE: Effect of superoxide radical on Ca2+ pumps of coronary artery. Am J Physiol 255: C297–C303, 1988

    PubMed  Google Scholar 

  14. Grover AK, Samson SE, Fomin VP: Peroxide inactivates calcium pumps in pig coronary artery. Am J Physiol 263: H537–H543, 1992

    PubMed  Google Scholar 

  15. Elmoselhi A, Samson S, Grover AK: SR Ca2+ pump heterogeneity in coronary artery: Free radicals and IP3-sensitive and-insensitive pools. Am J Physiol 271: C1652–C1659, 1996

    PubMed  Google Scholar 

  16. Elmoselhi A, Butcher A, Samson SE, Grover AK: Free radicals uncouple the sodium pump in pig coronary artery. Am J Physiol 266: C720–C728, 1994

    PubMed  Google Scholar 

  17. Grover AK, Samson SE, Lee RM: Subcellular fractionation of pig coronary artery smooth muscle. Biochim Biophys Acta 818: 191–199, 1985

    PubMed  Google Scholar 

  18. Grover AK, Samson SE, Fomin VP, Werstiuk ES: Effects of peroxide and superoxide on coronary artery: Ang II response and sarcoplasmic reticulum Ca2+-pump. Am J Physiol 269: C546–C553, 1995

    PubMed  Google Scholar 

  19. Balligand JL, Godfraind T: Effect of nisoldipine on contractions evoked by endothelin-1 in human isolated distal and proximal coronary arteries and veins. J Cardiovasc Pharmacol 24: 618–625, 1994

    PubMed  Google Scholar 

  20. Grover AK, Samson SE, Lee RM: Adenosine metabolism in small coronary arteries of pig. Biochemy Intl 27: 717–724, 1992

    Google Scholar 

  21. Heusch G, Deussen A, Schipke J, Thamer V: Alpha 1-and alpha 2-adrenoceptor-mediated vasoconstriction of large and small canine coronary arteries in vivo. J Cardiovasc Pharm 6: 961–968, 1984

    Google Scholar 

  22. Miwa K, Toda N: Regional differences in the response to vasoconstrictor agents of dog and monkey isolated coronary arteries. Brit J Pharmacol 82: 295–301, 1984

    Google Scholar 

  23. Xu XP, Liu Y, Tanner MA, Sturek M, Myers PR: Differences in nitric oxide production in porcine resistance arteries and epicardial conduit coronary arteries. J Cell Physiol 168: 539–548, 1996

    PubMed  Google Scholar 

  24. Misquitta C, Samson SE, Grover AK: Sarcoplasmic reticulum Ca2+-pump density is higher in distal than in proximal segments of porcine left coronary artery. Mol Cell Biochem 158: 91–95, 1996

    PubMed  Google Scholar 

  25. Gill DL, Ghosh TK, Bian J, Short AD, Waldron RT, Rybak SL: Function and organization of the inositol 1,4,5-trisphosphate-sensitive calcium pool. [Review]. Adv Sec Mess Phosphoprot Res 26: 265–308, 1992

    Google Scholar 

  26. Grover AK, Fomin VP, Samson SE: Angiotensin II contractions in coronary artery: Nature of receptors and calcium pools. Mol Cell Biochem 135: 11–19, 1994

    PubMed  Google Scholar 

  27. Gutterman DD, Rusch NJ, Hermsmeyer K, Dole WP: Differential reactivity to 5-hydroxytryptamine in canine coronary arteries. Blood Vess 23: 165–172, 1986

    Google Scholar 

  28. Demaurex N, Lew DP, Krause KH: Cyclopiazonic acid depletes intracellular Ca2+ stores and activates an influx pathway for divalent cations in HL-60 cells. J Biol Chem 267: 2318–2324, 1992

    PubMed  Google Scholar 

  29. Lytton J, Westlin M, Hanley MR: Thapsigargin inhibits the sarcoplasmic or endoplasmic reticulum Ca-ATPase family of calcium pumps. J Biol Chem 266: 17067–17071, 1991

    PubMed  Google Scholar 

  30. Seidler NW, Jona I, Vegh M, Martonosi A: Cyclopiazonic acid is a specific inhibitor of the Ca2+-ATPase of sarcoplasmic reticulum. J Biol Chem 264: 17816–17823, 1989

    PubMed  Google Scholar 

  31. Uyama Y, Imaizumi Y, Watanabe M: Cyclopiazonic acid, an inhibitor of Ca(2+)-ATPase in sarcoplasmic reticulum, increases excitability in ileal smooth muscle. Br J Pharmacol 110: 565–572, 1993

    PubMed  Google Scholar 

  32. Putney JW, Jr.: Receptor-regulated calcium entry. [Review]. Pharmacol Therap 48: 427–434, 1990

    Google Scholar 

  33. Vanbreemen C, Chen Q, Laher I: Superficial buffer barrier function of smooth muscle sarcoplasmic reticulum. Trends Pharmacol Sci 16: 98–105, 1995

    PubMed  Google Scholar 

  34. Missiaen L, Wuytack F, Raeyrnaekers L, de Smedt H, Droogmans G, Casteels R: Ca2+ extrusion across plasma membrane and Ca2+ uptake by intracellular stores. [Review]. Pharmacol Therap 50: 191–232, 1991

    Google Scholar 

  35. Ganitkevich VY, Isenberg G: Ca2+ entry through Na(+)-Ca2+ exchange can trigger Ca2+ release from Ca2+ stores in Na(+)-loaded guinea-pig coronary myocytes. J Physiol (Lond) 468: 225–243, 1993

    Google Scholar 

  36. Liu CY, Sturek M: Attenuation of endothelin-1-induced calcium response by tyrosine kinase inhibitors in vascular smooth muscle cells. Am J Physiol 270: C1825–C1833, 1996

    PubMed  Google Scholar 

  37. Ganitkevich VY, Isenberg G: Dissociation of subsarcolemnal from global cytosolic [Ca2+] in myocytes from guinea-pig coronary artery. J Physiol (Lond) 490: 305–318, 1996

    Google Scholar 

  38. Roveri A, Coassin M, Maiorino M, Zamburlini A, van Amsterdam FT, Ursini F: Effect of hydrogen peroxide on calcium homeostasis in smooth muscle cells. Arch Biochem Biophys 297: 265–270, 1992

    PubMed  Google Scholar 

  39. Toda N, Bian K, Inoue S: Age-related changes in the response to vasoconstrictor and dilator agents in isolated beagle coronary arteries. Arch Pharmacol 336: 359–364, 1987

    Google Scholar 

  40. Schilling WP, Elliott SJ: Ca2+ signaling mechanisms of vascular endothelial cells and their role in oxidant-induced endothelial cell dysfunction. [Review]. Am J Physiol 262: (Pt 2): H1617–H1630, 1992

    PubMed  Google Scholar 

  41. Doan TN, Gentry DL, Taylor AA, Elliott SJ: Hydrogen peroxide activates agonist-sensitive Ca2+-flux pathways in canine venous endothelial cells. Biochem J 297: 209–215, 1994

    PubMed  Google Scholar 

  42. Pantely GA, Bristow JD, Swenson LJ, Ladley HD, Johnson WM, Anselone CG: Incomplete coronary vasodilation during myocardial ischemia in swine. Am J Physiol 249: H638–H647, 1985

    PubMed  Google Scholar 

  43. Grover AK, Samson SE: Peroxide resistance of Ca2+ pump in endothelium in smooth muscle: Implications to coronary artery function. Am J Physiol 273: C1250–C1258, 1997

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada

    A.K. Grover, S.E. Samson, C.M. Misquitta & A.B. Elmoselhi

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  1. A.K. Grover
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  2. S.E. Samson
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  3. C.M. Misquitta
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  4. A.B. Elmoselhi
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Grover, A., Samson, S., Misquitta, C. et al. Effects of peroxide on contractility of coronary artery rings of different sizes. Mol Cell Biochem 194, 159–164 (1999). https://doi.org/10.1023/A:1006902603056

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