Abstract
Cyanogen bromide has been found to induce the template-guided condensation of oligonucleotides only in the presence of N-substituted morpholines. Based on 31 P,1 H and13 C NMR spectroscopy data, the mechanism of the phosphomonoester group activation by cyanogen bromide in N-substituted morpholine buffers is suggested. It has also been shown that BrCN can be used for the synthesis of oligonucleotide derivatives in aqueous solution.
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Shabarova, Z.A., Fedorova, O.A., Dolinnaya, N.G. et al. Derivatization and Template-Guided Ligation of Oligodeoxyribonucleotides Using Cyanogen Bromide and N-Substituted Morpholines. Orig Life Evol Biosph 27, 555–566 (1997). https://doi.org/10.1023/A:1006577107354
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DOI: https://doi.org/10.1023/A:1006577107354