Abstract
Preliminary work is needed to assess the usefulness of different markers at different taxonomic scales when a new group is analyzed, such as the commercially important Phytoseiidae mites. We investigate here the level of sequence variation of the nuclear ribosomal spacers ITS 1 and 2 and the 5.8S gene in six species of Phytoseiidae: Neoseiulus californicus, N. fallacis, Euseius concordis, Metaseiulus occidentalis, Typhlodromus pyri and Phytoseiulus persimilis. As expected, the 5.8S gene (148 base pairs) is markedly conserved and displays little variation in between genera comparisons. ITS1 and ITS2 show contrasting patterns: while the ITS2 is short (80–89 bp) and shows little variation, the ITS1 is longer (303–404 bp) and is very variable in sequence. This fact compromises reliable nucleotide homologies when comparing the genera. The comparison of ITS1 sequence similarity at the species level might be useful for species identification, however, the value of ITS in taxonomic studies does not extend to the level of the family. The intraspecific variations of ITS were investigated in three species: N. californicus, N. fallacis and E. concordis. The first species has identical ITS1 sequences and the last two display low polymorphism (2 nucleotide substitutions). The ITS2 and 5.8S sequences were identical in all three subspecies comparisons.
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Chant, D.A. and McMurtry, J.A. 1994. A review of the subfamilies Phytoseiinae and Typhlodrominae (Acari: Phytoseiidae). Internat. J. Acarol. 20: 223–310.
Chant, D.A. and Yoshida-Shaul, E. 1984. A world review of the occidentalis species group in the genus Typhlodromus Sheuten (Acarina: Phytoseiidae). Can. J. Zool. 62: 1860–1871.
Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package), Version 3.5p edition. Department of Genetics, University of Washington, Seattle.
Fenton, B., Malloch, G., Jones, A.T., Amrine Jr, J.W., Gordon, S.C., A'Hara, S., McGavin, W.J. and Birch, A.N.E. 1995. Species identification of Cecidophyopsis mites (Acari: Eriophyidae) from different Ribes species and countries using molecular genetics. Mol. Ecol. 4: 383–387.
Fritz, G.N., Conn, J., Cockburn, A. and Seawright, J. 1994. Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from a population of Anopheles nuneztovari (Diptera: Culicidae). Mol. Biol. Evol. 11: 406–416.
Gotoh, T., Gutierrez, J. and Navajas, M. 1998. Molecular comparison of the sibling species Tetranychus pueraricola Ehara & Gotoh and T. urticae Koch (Acari: Tetranychidae). Entomol. Sci. 1: 55–57.
Hillis, D.M. and Dixon, M.T. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Q. Rev. Biol. 66: 411–453.
Mangold, A.J., Bargues, M.D. and Mas-Coma, S. 1998. 18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: ixodidae). Parasitol. Res. 84: 31–37.
McMurtry, J.A. and Croft, B.A. 1997. Life styles of Phytoseiid mites and their role in biological control. Annu. Rev. Entomol. 42: 291–321.
Miller, B.R., Crabtree, M.B. and Savage, H.M. 1996. Phylogeny of fourteen Culex mosquito species, including the Culex pipiens complex, inferred from the internal transcribed spacers of ribosomal DNA. Insect Mol. Biol. 5: 93–107.
Monetti, L.N. and Croft, B.A. 1997. Mating, cross-mating and related behaviours of Neoseiulus californicus and Neoseiulus fallacis (Acari: Phytoseiidae). Exp. Appl. Acarol. 21: 67–74.
Moraes, G.J. de, McMurtry, J.A. and Denmark, H.A. 1986. A catalog of the mite family Phytoseiidae: references to taxonomy, synonymy, distribution and habitat. EMBRAPA-DDT, Brasilia.
Navajas, M., Gutierrez, J. and Gotoh, T. 1997. Convergence of molecular and morphological data reveals phylogenetic information in Tetranychus species and allows the restoration of the genus Amphitetranychus (Acari: Tetranychidae). Bull. Entomol. Res. 87: 283–288.
Navajas, M., Gutierrez, M., Lagnel, J. and Boursot, P. 1996. Mitochondrial cytochrome oxidase I in tetranychid mites: a comparison between molecular phylogeny and changes of morphological and life history traits. Bull. Entomol. Res. 86: 407–417.
Navajas, M., Lagnel, J., Gutierrez, J. and Boursot, P. 1998. Species wide homogeneity of nuclear ribosomal ITS2 sequences in the spider mite Tetranychus urticae contrasts with extensive mitochondrial COI polymorphism. Heredity 80: 742–752.
Paskewitz, S.M., Wesson, D.M. and Collins, F.H. 1993. The internal transcribed spacers of ribosonal DNA in five members of the Anopheles gambiae species complex. Insect Mol. Biol. 2: 247–257.
Schuster, R. and Pritchard, A.E. 1963. Phytoseiid mites of California. Hilgardia 34: 185–191.
Thompson, J.D., Higgins, D.G. and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through weighting, position-specific gap penalities and weight matrix. Nucleic Acids Res. 22: 4673–4680.
Wesson, D.M., McLain, D.K., Oliver, J.H., Piesman, J. and Collins, F.H. 1993. Investigation of the validity of species status of Ixodes dammini (Acari: ixodidae) using rDNA. Proc. Natl. Acad. Sci. U.S.A. 90: 10221–10225.
Wheeler, W.C., 1994. Sources of ambiguity in nucleic acid sequences alignment. In: Molecular Ecology and Evolution: Approaches and Applications, B. Schierwater, B. Streit, G.P. Wagner and R. DeSalle (eds), pp. 323–354. Birkhauser Verlag, Basel.
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Navajas, M., Lagnel, J., Fauvel, G. et al. Sequence Variation of Ribosomal Internal Transcribed Spacers (ITS) in Commercially Important Phytoseiidae Mites. Exp Appl Acarol 23, 851–859 (1999). https://doi.org/10.1023/A:1006251220052
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DOI: https://doi.org/10.1023/A:1006251220052