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Detection of Helicobacter-like Bacteria in Porcine Gastric Biopsy Samples by Amplification of 16S rRNA, ureB, vacA and cagA Genes by PCR

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Abstract

Preliminary evidence for the presence of Helicobacter-like bacteria was sought in 395 porcine gastric samples by a urease test. Of the samples, 37% (146/395) were urease-positive and 82% (82/100) of the Gram-stained urease-positive samples showed large, tightly spiralled organisms. Several methods were applied to culture the organisms but isolation was unsuccessful, contaminant organisms being considered to be one of the major problems. PCR with Helicobacter genus-specific primers for 16S rRNA and ureB genes, and primers for H. pylori vacA and cagA genes were tested with 102 urease-positive biopsy samples. The PCR results showed some evidence for the presence of the urease and the vacA genes in porcine Helicobacter-like bacteria and raises the possibility of pathogenicity by these organisms.

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REFERENCES

  • Andersen, L.P., Norgaard, A., Holck, S., Blom, J. and Elsborg, L., 1996. Isolation of a “Helicobacter heilmannii”-like organism from the human stomach. European Journal of Clinical Microbiology and Infectious Diseases, 15, 95-96

    Google Scholar 

  • Barbosa, A.J.A., Silva, J.C.P., Nogueira, A.M.M.F., Paulino, E. and Miranda, C.R., 1995. Higher incidence of Gastrospirillum sp. in swine with gastric ulcer of the pars oesophagea. Veterinary Pathology, 32, 134-139

    Google Scholar 

  • Bergann, Von T., 1991. Isolierung helikaler, campylobacterähnlicher Keime aus Schweinemägen. Monatshefte fur Veterinarmedizin, 46, 649-651

    Google Scholar 

  • Blaser, M.J., 1993. Helicobacter pylori: microbiology of a ‘slow’ bacterial infection. Trends in Microbiology, 1, 255-260

    Google Scholar 

  • Cover, T.L., Tummuru, M.K.R., Cao, P., Thompson, S.A. and Blaser, M.J., 1994. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. Journal of Biological Chemistry, 269, 10566-10573

    Google Scholar 

  • Grasso, G.M., Sammaro, M.L., Ripabelli, G., Ruberto, A. and Iannitto, G., 1995. In situ mapping of urease-positive areas in porcine gastric mucosa. Microbios, 82, 245-249

    Google Scholar 

  • Grasso, G.M., Ripabelli, G., Sammaro, M.L., Ruberto, A. and Iannitto, G., 1996. Prevalence of Helicobacter-like organisms in porcine gastric mucosa: a study of swine slaughtered in Italy. Comparative Immunology, Microbiology and Infectious Diseases, 19, 213-217

    Google Scholar 

  • Heilmann, K.L. and Borchard, F., 1991. Gastritis due to spiral shaped bacteria other than Helicobacter pylori: clinical, histological and ultrastructural findings. Gut, 32, 137-140

    Google Scholar 

  • Hänninen, M.-L., Happonen, I., Saari, S. and Jalava, K., 1996. Culture and characteristic of Helicobacter bizzozeronii, a new canine gastric Helicobacter sp. International Journal of Systematic Bacteriology, 46, 160-166

    Google Scholar 

  • Jalava, K., Kaartinen, M., Utriainen, M., Happonen, I. and Hänninen, M.-L., 1997. Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii. International Journal of Systematic Bacteriology, 47, 975-982

    Google Scholar 

  • Katelaris, P.H., Lowe, D.G., Norbu, P. and Farthing, J.G., 1992. Field evaluation of a rapid, simple and inexpensive urease test for the detection of Helicobacter pylori. Journal of Gastroenterology and Hepatology, 7, 569-571

    Google Scholar 

  • Korber-Golze, Von B. and Scupin, E., 1993. Helicobacter pylori: Untersuchungen beim Hausschwein. Deutsche TierarztlicheWochenschrift, 100, 465-468

    Google Scholar 

  • Mendes, E.N., Queiroz, D.M.M., Rocha, G.A., Moura, S.B., Leite, V.H.R. and Fonseca, M.E.F., 1990. Ultrastructure of a spiral micro-organism from pig gastric mucosa (“Gastrospirillum suis”). Journal of Medical Microbiology, 33, 61-66

    Google Scholar 

  • Mendes, E.N., Queiroz, D.M.M., Rocha, G.A., Nogueira, M.M.F., Carvalho, A.C.T., Lage, A.P. and Barbosa, A.J.A., 1991. Histopathological study of porcine gastric mucosa with and without a spiral bacterium (“Gastrospirillum suis”). Journal ofMedical Microbiology, 35, 345-348

    Google Scholar 

  • Phadnis, S.H., Ilver, D., Janzon, L., Normark, S. and Westblom, T.U., 1994. Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori. Infection and Immunity, 62, 1557-1565

    Google Scholar 

  • Queiroz, D.M.M., Rocha, G.A., Mendes, E.N., Lage, A.P., Carvalho, A.C.T. and Barbosa, A.J.A., 1990. A spiral microorganism in the stomach of pigs. Veterinary Microbiology, 24, 199-204

    Google Scholar 

  • Queiroz, D.M.M., Dewhirst, F.E., Paster, B.J., Carvalho, S.D., Magnago, A.G.P., Moura, S.B., Rocha, G.A. and Oliveira, A.M.R., 1995. Reservoir hosts for human “Gastrospirillum” infection. In: VIIIth International Workshop on Gastro-duodenal Pathology and Helicobacter pylori, July 1995, (European Helicobacter pylori Study Group, Gut), 103

  • Queiroz, D.M.M., Rocha, G.A., Mendes, E.N., De Moura, S.B., De Oliveira, A.M.R. and Miranda, D., 1996. Association between Helicobacter and gastric ulcer disease of the pars esophagea in swine. Gastroenterology, 111, 19-27

    Google Scholar 

  • Riley, L.K., Franklin, C.L., Hook, R.R. and Besch-Williford, C., 1996. Identification of murine Helicobacters by PCR and restriction enzyme analyses. Journal of Clinical Microbiology, 34, 942-946

    Google Scholar 

  • Solnick, J.V., O'Rourke, J., Lee, A. and Tompkins, L.S., 1994. Molecular analysis of urease genes from a newly identified uncultured species of Helicobacter. Infection and Immunity, 62, 1631-1638

    Google Scholar 

  • Stolte, M., Wellens, E., Bethke, B., Ritter, M. and Eidt, H., 1994. Helicobacter heilmannii (formerly Gastrospirillum hominis) gastritis - an infection transmitted by animals? Scandinavian Journal of Gastroenterology, 29, 1061-1064

    Google Scholar 

  • Tee, W., Lambert, J.R. and Dwyer, B., 1995. Cytotoxin production by Helicobacter pylori from patients with upper gastrointestinal tract diseases. Journal of Clinical Microbiology, 33, 1203-1205

    Google Scholar 

  • Tummuru, M.K.R., Cover, T.L. and Blaser, M.J., 1993. Cloning and expression of a high-molecular mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infection and Immunity, 61, 1799-1809

    Google Scholar 

  • Valentine, J.L., 1993. PCR detection of Helicobacter pylori. In: D.H. Persing, T.F. Smith, F.C. Temover and T.J. White (eds), 1993. Diagnostic Molecular Microbiology, (American Society for Microbiology, Washington DC), 282-287

    Google Scholar 

  • Vaira, D., Holton, J., Londe, M., Beltrandi, E., Salmon, P.R., D'Anastasio, C., Dowsett, J.F., Bertoni, F., Grauenfels, P. and Gandolfi, L., 1988. Campylobacter pylori in abattoir workers: is it a zoonosis? Lancet, ii, 725-726

    Google Scholar 

  • Xiang, Z., Censini, S., Bayeli, P.F., Telford, J.L., Figura, N., Rappuoli, R. and Covacci, A., 1995. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infection and Immunity, 63, 94-98

    Google Scholar 

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Authors and Affiliations

  1. Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, PO Box 57, 00014, Helsinki, Finland

    M. Utriainen & M.-L. Hänninen

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  1. M. Utriainen
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  2. M.-L. Hänninen
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Utriainen, M., Hänninen, ML. Detection of Helicobacter-like Bacteria in Porcine Gastric Biopsy Samples by Amplification of 16S rRNA, ureB, vacA and cagA Genes by PCR. Vet Res Commun 22, 373–384 (1998). https://doi.org/10.1023/A:1006141211452

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