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Bnm1, a Brassica pollen-specific gene

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Abstract

cDNA and genomic clones of a new pollen-specific gene, Bnm1, have been isolated from Brassica napus cv. Topas. The gene contains an open reading frame of 546 bp and a single intron of 362 bp. A comparison of the deduced amino acid sequence with sequences in data banks did not show similarity with known proteins. Northern blot analysis of developing pollen showed that Bnm1 mRNA was first detected in bicellular pollen and accumulated to higher levels in tricellular pollen. Bnm1 mRNA was not detected in leaves, stems, roots, pistils, seeds or pollen-derived embryos. RNA in situ hybridization of whole flower buds confirmed that Bnm1 was pollen-specific and expressed late in development. A promoter fragment of the Bnm1 gene fused to the gusA reporter gene yielded similar patterns of tissue specificity and developmental regulation in transgenic B. napus cv. Westar plants; however, the promoter was also active during the early stages of pollen development. The Bnm1 gene, cloned in this study, was derived from the A genome of the allotetraploid species B. napus (AACC). Southern blot analysis indicated that sequences similar to the Bnm1 gene were found in both A and C Brassica genomes. Related sequences were found in all 10 members of the Brassiceae tribe examined, but were not present in all tribes of the Brassicaceae family.

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Authors and Affiliations

  1. Ottawa-Carleton Institute of Biology, University of Ottawa, Ottawa, Ontario, Canada, K1N 6N5

    Brian K. Treacy & Douglas A. Johnson

  2. Eastern Cereal and Oilseed Research Center, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada, K1A OC6

    Jiro Hattori, Kim Boutilier & Brian L. Miki

  3. Pioneer Hi-Bred Production Ltd, Mississauga, Ontario, Canada, L7G 4S7

    Isabelle Prud'homme, Eric Barbour & Bin Huang

  4. Trait and Technology Division, Pioneer Hi-Bred Production International Inc., Johnston, IO, USA, 50131-1004

    Chris L. Baszczynski

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  1. Brian K. Treacy
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  2. Jiro Hattori
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  3. Isabelle Prud'homme
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  4. Eric Barbour
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  5. Kim Boutilier
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  6. Chris L. Baszczynski
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  7. Bin Huang
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  8. Douglas A. Johnson
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  9. Brian L. Miki
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Treacy, B.K., Hattori, J., Prud'homme, I. et al. Bnm1, a Brassica pollen-specific gene. Plant Mol Biol 34, 603–611 (1997). https://doi.org/10.1023/A:1005851801107

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  • DOI: https://doi.org/10.1023/A:1005851801107

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