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The use of succinic acid, as a pH buffer, expands the potentialities of utilisation of a chemically defined medium in Saccharomyces cerevisiae flocculation studies

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Abstract

The pH, viability and flocculation during the growth of Saccharomyces cerevisiae NCYC 1214 in rich medium (YEPD) and in chemically defined medium (YNB) were compared. As the pH fell from 5.3 to 2.2, the viability fell to 20% and no flocculation was observed in YNB medium, despite the high flocculation in buffer. With the addition of 50 mM succinic acid and 3.1 mM Ca2+, similar growth to YEPD, with 99% of viability and flocculation in culture medium, was obtained.

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Authors and Affiliations

  1. Departamento de Engenharia Química, Instituto Superior de Engenharia do Instituto Politécnico do Porto, CIEA-, Rua Dr António Bernardino de Almeida, 431, P-4200-072, Porto, Portugal

    Eduardo V. Soares & Jan Seynaeve

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  1. Eduardo V. Soares
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  2. Jan Seynaeve
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Soares, E.V., Seynaeve, J. The use of succinic acid, as a pH buffer, expands the potentialities of utilisation of a chemically defined medium in Saccharomyces cerevisiae flocculation studies. Biotechnology Letters 22, 859–863 (2000). https://doi.org/10.1023/A:1005667431344

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  • DOI: https://doi.org/10.1023/A:1005667431344

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