Abstract
To determine the transmission route of Helicobacter pylori, one nude mouse was challenged by H. pylori, and then raised with nonchallenged nude mice in a single cage in a sterilized environment with and without exposure to their feces. After coraising for two and four weeks, all mice were killed to determine H. pylori in the stomach, saliva, and feces and to assess gastritis grade. Natural transmission of H. pylori occurred in 50% (2/4) and 70% (7/10) of mice after two weeks and four weeks of coraising when they were exposed to their feces. H. pylori was detected not only in the stomach but also in saliva and feces by PCR of all challenged and transmitted mice. However, no transmission occurred in mice not exposed to feces of a challenged mouse, while sharing food and water in a single cage. These findings suggest that the fecal–oral transmission route is important, at least in the animal model. Serum levels of anti-H. pylori urease IgG of the H. pylori-transmitted mice increased after coraising, and gastritis was observed in the stomach of both challenged and transmitted mice. We conclude that H. pylori bacteria are transmitted through the fecal–oral route from challenged to nonchallenged nude mice, resulting in gastritis.
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REFERENCES
Graham DY, Alpert LC, Smith JL, Yoshimura HH: Iatrogenic Campylobacter pylori infection is a case of epidemic achlorhydria. Am J Gastroenterol 83:974-980, 1988
Blaser MJ: Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 161:626-633, 1990
Alam K, Schubert TT, Bologna SD, Ma CK: Increased density of Helicobacter pylori on antral biopsy is associated with severity of acute and chronic inflammation and likelihood of duodenal ulceration. Am J Gastroenterol 87:424-428, 1992
Tytgat GNJ: Helicobacter pylori infection and duodenal ulcer disease. J Int Med 231:199-203, 1992
Matsumoto S, Washizuka Y, Matsumoto Y, Tawara S, Ikeda F, Yokota Y, Karita M: Induction of ulceration and severe gastritis in Mongolian gerbil by Helicobacter pylori infection. J Med Microbiol 46:391-397, 1997
Sugiyama A, Maruta F, Ikeno T, Ishida K, Kawasaki S, Katsuyama T, Shimizu N, Tatematsu M: Helicobacter pylori infection enhances N-methyl-N-nitrosourea-induced stomach carcinogenesis in the Mongolian gerbil. Cancer Res 58:2067-2069, 1998
Watanabe T, Tada M, Nagai H, Sasaki S, Nakao M: Helicobacter pylori infection induces gastric cancer in Mongolian gerbils. Gastroenterology 115:642-648, 1998
Graham DY, Malaty HM, Evans DG, Evans DJJ, Klein PD, Adam E: Epidemiology of Helicobacter pylori in an asymptomatic population in the United States. Gastroenterol 100:1495-1501, 1991
Koster T, Vandenbroucke JP: Helicobacter pylori, musings from the epidemiologic armchair. Epidemiol Infect 109:81-85, 1992
Ferguson DA JR, Li C, Patel NR, Mayberry WR, Chi DS, Thomas E: Isolation of Helicobacter pylori from saliva. J Clin Microbiol 31:2802-2804, 1993
Karajden S, Fuksa M, Anderson J, Kempston J, Boccia A, Petrea C, Babida C, Karmali M, Penner JL: Examination of human stomach biopsies, saliva, and dental plaque for Campylobacter pylori. J Clin Microbiol 27:1397-1398, 1989
Thomas JE, Gibson GR, Darboe MK, Dale A, Weaver LT: Isolation of Helicobacter pylori from human faeces. Lancet 340:1194-1195, 1992
Lee A, Fox JG, Otto G, Dick EH, Krakowka S: Transmission of Helicobacter spp., a challenge to the dogma of faecal-oral spread. Epidemiol Infect 107:99-109, 1991
Karita M, Kouchiyama T, Okita K, Nakazawa T: New small animal model for human gastric Helicobacter pylori infection: Success in both nude and euthymic mice. Am J Gastroenterol 86:1596-1603, 1991
Enroth H, Engstrand L: Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens. J Clin Microbiol 33:2162-2165, 1995
Clayton CL, Kleanthous H, Coates PJ, Morgan DD, Tabaqchali S: Sensitive detection of Helicobacter pylori by using polymerase chain reaction. J Clin Microbiol 30:192-200, 1992
Kawamata O, Yoshida H, Hirota K, Yoshida A, Kawaguchi R, Shiratori Y, Omata M: Nested-polymerase chain reaction for the detection of Helicobacter pylori infection with novel primers designed by sequence analysis of urease A gene in clinically isolated bacterial strains. Biochem Biophys Res Commun 219:266-272, 1996
Nagata K, Mizuta T, Tonokatu Y, Fukuda Y, Okamura H, Hayashi T, Shimoyama T, Tamura T: Monoclonal antibodies against the native urease of Helicobacter pylori: Synergistic inhibition of urease activity by monoclonal antibody combinations. Infect Immun 60:4826-4831, 1992
Karita M, Li Q, Cantero D, Okita K: Establishment of a small animal model for human Helicobacter pylori infection using germ-free mouse. Am J Gastroenterol 89:208-213, 1994
Price AB: The Sydney system: Histological division. J Gastroenterol Hepatol 6:209-222, 1991
Chan WY, Hui PK, Leung KM, Chow J, Kwok F, Chi-Sing NG: Coccoid forms of Helicobacter pylori in the human stomach. Am J Clin Pathol 102:503-507, 1994
Cellini L, Allocati N, Angelucci D, Iezzi T, Campli ED, Marzio L, Dainelli B: Coccoid Helicobacter pylori not culturable in vitro reverts in mice. Microbiol Immunol 38(11):843-850, 1994
Lee A, O'Rourke J, De Ungria MC, Robertson B, Daskalopoulos G, Dixon MF: A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strain. Gastroenterol 112:1386-1397, 1997
Drumm B, Perez-Perez GI, Blaser MJ, Sherman PM: Intrafamilial clustering of Helicobacter pylori infection. N Engl Med 322:359-363, 1990
Lambert JR, Lin SK, Nicholson L, Sievert W, Sharma V, Kaldor J, Coulepis AG: High prevalence of H. pylori antibodies in institutionalized adults. Gastroenterology 98:A74, 1990 (abstract)
Marshall BJ, Armstrong JA, McGechie DB, Glancy RJ: Attempt to fulfil Koch's postulates for pyloric campylobacter. Med J Aust 142:436-439, 1985
Morris A, Lane M, Hamilton I, Samarasinghe D, Ali MR, Brown P, Nicholson G: Duodenal ulcer relapse after eradication of Helicobacter pylori. NZ Med J 104:329-331, 1991
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Yoshimatsu, T., Shirai, M., Nagata, K. et al. Transmission of Helicobacter pylori from Challenged to Nonchallenged Nude Mice Kept in a Single Cage. Dig Dis Sci 45, 1747–1753 (2000). https://doi.org/10.1023/A:1005586312582
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DOI: https://doi.org/10.1023/A:1005586312582