Abstract
The roots of most plants form symbiotic associations with mycorrhizal fungi. The net flux of nutrients, particularly phosphorus (P), from the soil into the plant is greater in mycorrhizal than in comparable non-mycorrhizal plants. However despite the widespread occurrence of mycorrhizal associations the processes controlling the transfer of solutes between the symbionts are poorly understood. To understand the mechanisms regulating the transfer of solutes information about conditions at the interface between plant and fungus is needed.
Measurements of apoplastic and intracellular electrical potential difference in leek roots colonised by mycorrhizal fungi and estimates of cytosolic pH in fungal hyphae are presented. These and the implications for plant/fungal mineral nutrition in vesicular-arbuscular mycorrhizas are discussed.
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References
Ashford A E, Allaway W G, Peterson C A and Cairney J W G 1989 Nutrient transfer and the fungus-root interface. Aust. J. Plant Physiol. 16, 85-97.
Ayling S M, Smith S E and Smith F A (1996) The physiology of the plant-mycorrhizal fungus interface: preliminary observations. InProceedings, First International Conference on Mycorrhizae, Berkeley, California, USA. p 22.
Bonfante-Fasolo P 1984 Anatomy and morphology of VA mycorrhiza. InV A Mycorrhiza. Eds. C L Powell and D J Bagyaraj. pp 5-31. CRC Press, Boca Raton, Florida, USA.
Bonfante-Fasolo P and Grippiolo R 1982 Ultrastructural and cytochemical changes in the wall of a vesicular-arbuscular mycorrhizal fungus during symbiosis. Can. J. Bot. 60, 2303-2312.
Clarkson D T 1985 Factors affecting mineral nutrient acquisition by plants. Annu. Rev. Plant Physiol. 36, 77-115.
Cork R J 1986 Problems with the application of quin-2-AM to measuring cytoplasmic free calcium in plant cells. Plant Cell Environ. 9, 157-161.
Cox G and Tinker P B 1976 Translocation and transfer of nutrients in vesicular-arbuscular mycorrhizas. I The arbuscule and phosphorus transfer: a quantitative ultrastructural study. New Phytol. 77, 371-378.
Fieschi M, Alloatti G, Sacco S and Berta G 1992 Membrane potential hyperpolarisation in vesicular arbuscular mycorrhizae of Allium porrumL.: a non-nutritional long-distance effect of the fungus. Protoplasma 168, 136-140.
Gianinazzi-Pearson V, Smith S E, Gianinazzi S and Smith F A 1991 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhizas V. Is H+ATPase a component of ATP-hydrolysing enzyme activities in plant-fungus interfaces? New Phytol. 117, 61-74.
Grignon C and Sentenac H 1991 pH and ionic conditions in the apoplast. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 103-28.
Harrison M J and van Buuren M L (1995) A phosphate transporter from the mycorrhizal fungus Glomus versiforme. Nature 378, 626-629.
Jensen A 1982 Influence of four vesicular arbuscular mycorrhizal fungi on nutrient uptake and growth in barley (Hordeum vulgare). New Phytol. 90, 45-50.
Jolicoeur M, Germette S, Gaudette M, Perrier M and Becard G 1996 Measurement of endomycorrhizal fungi intracellular pH. InProceedings, First International Conference on Mycorrhizae, Berkeley, California, USA. p 67.
Kurkdjian A and Guern J 1989 Intracellular pH: measurement and importance in cell activity. Ann. Rev. Plant Physiol. Plant Mol. Biol. 40, 271-303.
Marx C, Dexheimer T, Gianinazzi-Pearson V and Gianinazzi S 1982 Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhizas IV. Ultracytoenzymological evidence (ATPase) for active transfer in the host arbuscule interface. New Phytol. 90, 37-43.
Mimura T 1995 Homeostasis of inorganic phosphate in plants. Plant Cell Physiol. 36, 1-7.
Murphy P J, Langridge P and Smith S E 1996 Cloning plant genes differentially expressed during colonisation of roots of Hordeum vulgareby the vesicular-arbuscular mycorrhizal fungus Glomus intraradices. New Phytol. 135, 291-301.
Oparka K J and Read N D 1994 The use of fluorescent probes for studies of living plant cells. InPlant Cell Biology. Eds. N Harris and K J Oparka. pp 27-50. IRL Press, Oxford, UK.
Purves R D 1981 Microelectrode methods for intracellular recording and ionphoresis. Academic Press, London, UK p146.
Robson G D, Prebble E, Rickers A, Hosking S, Denning D W, Trinci A P J and Robertson W 1996 Polarised growth of fungal hyphae is defined by an alkaline pH gradient. Fungal Genetics and Biology 20, 289-298.
Rost F W, Shepherd V A and Ashford A E 1995 Estimation of vacuolar pH in actively growing hyphae of the fungus Pisolithus tinctorius. Mycol. Res. 99, 549-553.
Smith F A and Smith S E 1995 Nutrient transfer in vesicular-arbuscular mycorrhizas: a new model based on the distribution of ATPases on fungal and plant membranes. Biotropia 8, 1-10.
Smith F A and Smith S E 1997 Structural diversity in (Vesicular) — Arbuscular Mycorrhizal symbioses. New Phytol. (in press).
Smith S E and Read D J 1997 Mycorrhizal Symbiosis Academic Press, London. 605p.
Smith S E and Smith F A. 1990 Structure and function of the interfaces in biotrophic symbioses as they relate to nutrient transport. New Phytol. 114, 1-38.
Smith S E, Dickson S, Morris C and Smith F A 1994 Transfer of phosphate from fungus to plant in VA mycorrhizas: calculation of the area of symbiotic interface and of fluxes of P from two different fungi to Allium porrumL. New Phytol. 127, 93-99.
Thomson B D, Clarkson D T and Brain P 1990 Kinetics of phosphorus uptake by the germ-tubes of the vesicular-arbuscular fungus Gigaspora margarita. New Phytol. 116, 647-653.
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Ayling, S.M., Smith, S.E., Smith, F.A. et al. Transport processes at the plant-fungus interface in mycorrhizal associations: physiological studies. Plant and Soil 196, 305–310 (1997). https://doi.org/10.1023/A:1004284326231
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DOI: https://doi.org/10.1023/A:1004284326231