Skip to main content
SpringerLink
Log in

Changes in Manganese Superoxide Dismutase Expression After Exposure of the Retina to Intense Light

  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Abstract

Manganese superoxide dismutase (Mn-SOD) is a naturally-occurring scavenger of superoxide, one of several reactive oxygen intermediates. To determine if Mn-SOD expression is enhanced as a defensive mechanism against oxidative challenges, such as intense light exposure, rats were exposed to cyclic light (80 lux) for 2 weeks, intense light (1,800 lux) for 24 h, and then again to cyclic light. Experimental and control (exposed to cyclic light only) eyes were enucleated 3 h, 1, 3, 7, and 14 days after light challenge. Protein expression was examined immunohistochemically using rabbit antisera against rat Mn-SOD. There was no significant difference between the light-exposed and the control groups in the thickness of the outer nuclear layers. Both retinal pigment epithelial cells and photoreceptor inner segments in the normal retina were labeled for Mn-SOD. Mn-SOD labeling was lost 3 h and day 1 after light challenge. It was re-expressed in the retinal pigment epithelial cells 3, 7, and 14 days after the light challenge, and in the photoreceptor inner segments after day 14. These results suggest that the retina might have a protective potential against light damage, in which Mn-SOD may play an important role.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References cited

  • Akai F, Maeda M, Suzuki K, Inagaki S, Takagi H, Taniguchi N (1990) Immunocytochemical localization of manganese superoxide dismutase (Mn-SOD) in the hippocampus of the rat. Neurosci Lett 115: 19–23.

    Google Scholar 

  • Asayama K, Burr IM (1985) Rat superoxide dismutases. Purification, labeling, immunoassay, and tissue concentration. J Biol Chem 260: 2212–2217.

    Google Scholar 

  • Asoh K, Watanabe Y, Mizoguchi H, Mawatari M, Ono M, Kohno K, Kuwano M (1989) Induction of manganese superoxide dismutase by tumor necrosis factor in human breast cancer MCF-7 cell line and its TNF-resistant variant. Biochem Biophy Res Commun 162: 794–801.

    Google Scholar 

  • de la Paz MA, Zhang J, Fridovich I (1996) Antioxidant enzymes of the human retina: effect of age on enzyme activity of macula and periphery. Curr Eye Res 15: 273–278.

    Google Scholar 

  • del Vecchio PJ, Shaffer JB (1991) Regulation of antioxidant enzyme expression in LPS-treated bovine retinal pigment epithelial and corneal endothelial cells. Curr Eye Res 10: 919–925.

    Google Scholar 

  • Elner VM, Strieter RM, Elner SG, Baggiolini M, Lindley I, Kunkel SL (1990) Neutrophil chemotactic factor (IL-8) gene expression by cytokine-treated retinal pigment epithelial cells. Am J Pathol 136: 745–750.

    Google Scholar 

  • Elner SG, Strieter RM, Elner VM, Rollins BJ, del Monte MA, Kunkel SL (1991) Monocyte chemotactic protein gene expression by cytokinetreated human retinal pigment epithelial cells. Lab Invest 64: 819–825.

    Google Scholar 

  • Faktorovich EG, Steinberg RH, Yasumura D, Matthes MT, la Vai MM (1992) Basic fibroblast growth factor and local injury protect photoreceptors from light damage in the rat. J Neurisci 12: 3554–3567.

    Google Scholar 

  • Feeney L, Berman ER (1976) Oxygen toxicity: membrane damage by free radicals. Invest Ophthalmol 15: 789–792.

    Google Scholar 

  • Garcia JH, Anderson ML (1989) Physiopathology of cerebral ischemia. Crit Rev Neurobiol 4: 303–324.

    Google Scholar 

  • Hagege J, Delarue F, Peraldi MN, Sraer JD, Rondeau E (1994) Heparin selectively inhibits synthesis of tissue type plasminogen activator and matrix deposition of plasminogen activator inhibitor 1 by human mesangial cells. Lab Invest 71: 828–837.

    Google Scholar 

  • Halliwell B (1992) Reactive oxygen species and the central nervous system. J Neurochem 59: 1609–1623.

    Google Scholar 

  • Holley JA, Janssen YM, Mossmann BT, Taatjes DJ (1992) Increased manganese superoxide dismutase protein in type II epithelial cells of rat lungs after inhalation of crocidolite asbestos or cristobalite silica. Am J Pathol 141: 475–485.

    Google Scholar 

  • Li L, Turner JE (1991) Optimal conditions for long-term photoreceptor cell rescue in RCS rats: the necessity for healthy RPE transplants. Exp Eye Res 52: 669–679.

    Google Scholar 

  • Lucius R, Sievers J (1996) Postnatal retinal ganglion cells in vitro: protection against reactive oxygen species (ROS)-induced axonal degeneration by cocultured astrocytes. Brain Res 743: 56–62.

    Google Scholar 

  • Munim A, Asayama K, Dobashi K, Suzuki K, Kawaoi A, Kato K (1992) Immunohistochemical localization of superoxide dismutases in fetal and neonatal rat tissues. J Histochem Cytochem 40: 1705–1713.

    Google Scholar 

  • Newsome DA, Dobard EP, Liles MR, Oliver PD (1990) Human retinal pigment epithelium contains two distinct species of superoxide dismutase. Invest Ophthalmol Vis Sci 31: 2508–2513.

    Google Scholar 

  • Noell WK (1980) There are different kinds of retinal light damage. In: The effects of constant light on visual processes (Ed.Williams TP and Baker BN) pp. 3–28, Plenum, New York, USA.

    Google Scholar 

  • Ohira A, Honda O, Gauntt CD, Yamamoto M, Hori K, Masutani H, Yodoi J, Honda Y (1994) Oxidative stress induces adult T cell leukemia derived factor/thioredoxin in the rat retina. Lab Invest 70: 279–285.

    Google Scholar 

  • Oliver PD, Newsome DA (1992) Mitochondrial superoxide dismutase in mature and developing human retinal pigment epithelium. Invest Ophthalmol Vis Sci 33: 1909–1918.

    Google Scholar 

  • Organisciak DT, Darrow RM, Jiang YL, Blanks JC (1996) Retinal light damage in rats with altered levels of rod outer segment docosahexaenoate. Invest Ophthalmol Vis Sci 37: 2243–2257.

    Google Scholar 

  • Organisciak DT, Wang HM, Xie A, Reeves DS, Donoso LA (1989) Intense-light mediated changes in rat rod outer segment lipids and proteins. Prog Clin Biol Res 314: 493–512.

    Google Scholar 

  • Organisciak DT, Wang HM, Kou AL (1984) Ascorbate and glutathione levels in the developing normal and dystrophic rat retina: effect of intense light exposure. Curr Eye Res 3: 57–67.

    Google Scholar 

  • Park CM, Hollenberg MJ (1989) Basic fibroblast growth factor induces retinal regeneration in vivo. Dev Biol 134: 201–205.

    Google Scholar 

  • Park CM, Hollenberg MJ (1991) Induction of retinal regeneration in vivo by growth factors. Dev Biol 148: 322–333.

    Google Scholar 

  • Penn JS, Anderson RE (1987) Effect of light history on rod outer-segment membrane composition in the rat. Exp Eye Res 44: 767–778.

    Google Scholar 

  • Penn JS, Naash MI, Anderson RE (1987) Effect of light history on retinal antioxidants and light damage susceptibility in the rat. Exp Eye Res 44: 779–788.

    Google Scholar 

  • Reme CE, Braschler UF, Roberts J, Dillon J (1991) Light damage in the rat retina: effect of a radioprotective agent (WR-77913) on acute rod outer segment disk disruptions. Photochem Photobiol 54: 137–142.

    Google Scholar 

  • Szabo ME, Droy-Lefaix MT, Doly M, Braquest P (1992) Ischaemiaand reperfusion-induced Na+, K+, Ca2+ and Mg2+ shifts in rat retina: effects of two free radical scavengers, SOD and EGB 761. Exp Eye Res 55: 39–45.

    Google Scholar 

  • Shiki Y, Meyrick BO, Brigham KL, Burr IM (1987) Endotoxin increases superoxide dismutase in cultured bovine pulmonary endothelial cells. Am J Physiol 252: 436–440.

    Google Scholar 

  • Slot JW, Geuze HJ, Freeman BA, Crapo JD (1986) Intracellular localization of the copper-zinc and manganese superoxide dismutases in rat liver parenchymal cells. Lab Invest 55: 363–371.

    Google Scholar 

  • Summers RW, Maves BV, Reeves RD, Arjes LJ, Oberley LW (1989) Irradiation increases superoxide dismutase in rat intestinal smooth muscle. Free Rad Biol Med 6: 261–270.

    Google Scholar 

  • Tajima Y, Kato K, Maruyama S, Hosoi K (1996) In vivo modulation of proliferating cell nuclear antigen in growth plate chondrocytes from normal, hypophysectomized, growth hormone-treated hypophysectomized rats: a comparative immunohistochemical study with image analysis. J Histochem Cytochem 44: 713–720.

    Google Scholar 

  • Tamura Y, Chi LG, Driscoll EM Jr, Hoff PT, Freeman BA, Gallagher KP, Lucchesi BR (1988) Superoxide dismutase conjugated to polyethylene glycol provides sustained protection against myocardial ischemia/reperfusion injury in caine heart. Circ Res 63: 944–959.

    Google Scholar 

  • Tang G, Berg JT, White JE, Lumb PD, Lee CY, Tsan MF (1994) Protection against oxygen toxicity by tracheal insufflation of endotoxin: role of Mn SOD and alveolar macrophages. Am J Physiol 266: L38–L45.

    Google Scholar 

  • Warner BB, Burhans MS, Clark JC, Wipspe JR (1991) Tumor necrosis factor-alpha increases Mn-SOD expression: protection against oxidant injury. Am J Physiol 260: L296–L301.

    Google Scholar 

  • White CW, Ghezz, P., McMahon S, Dinarello CA, Repine JE (1989) Cytokines increase rat lung antioxidant enzymes during exposure to hyperoxia. J Appl Physiol 66: 1003–1007.

    Google Scholar 

  • Wiechmann AF, O'Steen WK (1992) Melatonin increases photoreceptor susceptibility to light-induced damage. Invest Ophthalmol Vis Sci 33: 1894–1902.

    Google Scholar 

  • Wiegand RD, Giusto NM, Rapp LM, Anderson RE (1983) Evidence for rod outer segment lipid peroxidation following constant illumination of the rat retina. Invest Ophthalmol Vis Sci 24: 1433–1435.

    Google Scholar 

  • Wilson CA, Hatchell DL (1991) Photodynamic retinal vascular thrombosis. Invest Ophthalmol Vis Sci 32: 2357–2365.

    Google Scholar 

  • Wispe JR, Warner BB, Clark JC, Dey CR, Neuman J, Glasser SW, Crapo JD, Chang LY, Whitsett JA (1992) Human Mn-superoxide dismutase in pulmonary epithelial cells of transgenic mice confers protection from oxygen injury. J Biol Chem 267: 23937–23941.

    Google Scholar 

  • Yamamoto M, Ohira A, Honda O, Sato N, Furuke K, Yodoi J, Honda Y (1997) Analysis of localization of adult T-cell leukemia-derived factor in the transient ischemic rat retina after treatment with OP-1206 alpha-CD, a prostaglandin E1 analogue. J Histochem Cytochem 45: 63–70.

    Google Scholar 

  • Yamashita H, Horie K, Yamamoto T, Katagiri H, Asano T, Hirano T, Nagano T, Oka Y (1994) Superoxide dismutase in developing mouse retina. Jpn J Ophthalmol 38: 148–161.

    Google Scholar 

  • Yamashita H, Horie K, Yamamoto T, Nagano T, Hirano T (1992) Lightinduced retinal damage in mice. Hydrogen peroxide production and superoxide dismutase activity in retina. Retina 12: 59–66.

    Google Scholar 

  • Zhang J, Piantadosi CA (1992) Mitochondrial oxidative stress after carbon monoxide hypoxia in the rat brain. J Clin Invest 90: 1193–1199.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Miho Yamamoto

  2. Department of Ophthalmology, Nagasaki University School of Medicine, Nagasaki, Japan

    Kria Lidia, Huaqing Gong, Setsuko Onitsuka & Takayo Kotani

  3. Department of Ophthalmology, Shimane Medical University, Japan

    Akihiro Ohira

Authors
  1. Miho Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kria Lidia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Huaqing Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Setsuko Onitsuka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takayo Kotani
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Akihiro Ohira
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamamoto, M., Lidia, K., Gong, H. et al. Changes in Manganese Superoxide Dismutase Expression After Exposure of the Retina to Intense Light. Histochem J 31, 81–87 (1999). https://doi.org/10.1023/A:1003510719302

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1003510719302

Keywords

Search

Navigation