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Ascospore aggregation and oxylipin distribution in the yeast Dipodascopsis tothii

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Abstract

Upon cultivation of the yeast Dipodascopsis tothii in its sexual stage, small ascospores are released individually from the ascus tip, which then assemble in sheathed cluster balls. In contrast to Dipodascopsis uninucleata, this yeast produced smooth bean shaped ascospores with sheath-like appendages that assemble in a disordered sheathed ball of ascospores outside the ascus. Strikingly, upon release, the ascus tip contained 3-hydroxy oxylipins, while the released ascospore clusters contained little or no 3-hydroxy oxylipins as indicated by immunofluorescence microscopy. In D. uninucleata, these oxylipins are concentrated on the spore surface and interspore matrix, but not on the ascus tip.

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Authors and Affiliations

  1. Lipid Biotechnology Group, Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, Bloemfontein, 9301, South Africa

    D.P. Smith, M.I. Motaung, P. Venter & D.J. Coetzee

  2. Lipid Biotechnology Group, Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, Bloemfontein, 9301, South Africa

    J.L.F. Kock

  3. Electron Microscope Unit, Department of Botany and Genetics, University of the Orange Free State, P.O. Box 339, Bloemfontein, 9301, South Africa

    P.W.J. van Wyk

  4. Eicosanoid Research Division, Department of Gynaecology, University Medical Centre Benjamin Franklin, Free University Berlin, D-12200, Berlin, Germany

    S. Nigam

Authors
  1. D.P. Smith
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  2. J.L.F. Kock
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  3. M.I. Motaung
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  4. P.W.J. van Wyk
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  5. P. Venter
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  6. D.J. Coetzee
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  7. S. Nigam
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Correspondence to J.L.F. Kock.

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Smith, D., Kock, J., Motaung, M. et al. Ascospore aggregation and oxylipin distribution in the yeast Dipodascopsis tothii. Antonie Van Leeuwenhoek 77, 389–392 (2000). https://doi.org/10.1023/A:1002789025872

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  • DOI: https://doi.org/10.1023/A:1002789025872

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