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Transfer of plasmid RP4 in the spermosphere and rhizosphere of barley seedling

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Abstract

Transfer of plasmid RP4 to indigenous bacteria in bulk soil could only be detected in soil with nutrient amendment. Lack of physiological active donor and recipient cells was apparently one of the limiting factors in un-amended bulk soil. Plasmid transfer was detected both in the spermosphere and rhizosphere of barley seedlings. Transfer occured from seed coated donor bacteria (i) to introduced recipient bacteria and (ii) to indigenous bacteria present in soil. Plasmid transfer was also detected from donor bacteria introduced to the soil to seed coated recipient bacteria. Transfer efficiencies in the rhizosphere were significantly below the transfer efficiencies obtained in the spermosphere. The transfer efficiencies detected in the barley spermosphere were among the highest reported from any natural environment.

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Authors and Affiliations

  1. Department of General Microbiology, Institute of Molecular Biology, University of Copenhagen, S$oring;lvgade 83 H, DK1307, Copenhagen K, Denmark

    Søren J. Sørensen & Linda Elise Jensen

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  1. Søren J. Sørensen
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  2. Linda Elise Jensen
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Correspondence to Søren J. Sørensen.

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Sørensen, S.J., Jensen, L.E. Transfer of plasmid RP4 in the spermosphere and rhizosphere of barley seedling. Antonie Van Leeuwenhoek 73, 69–77 (1998). https://doi.org/10.1023/A:1000661115753

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