Abstract
We studied the effects of auxin (indole-3-acetic acid) on formation of the primary polarity axis in zygotes of the brown algae Fucus vesiculosusL. Within the first 2.5 h after fertilization, the zygotes release this phytohormone in the ambient medium. The treatment of developing zygotes with the inhibitor of indole-3-acetic acid transport from the cell 2,3,5-triiodobenzoic acid at 5 mg/l arrests the auxin secretion and leads to its accumulation in the cells. This causes a significant delay in zygote polarization. The treatment of zygotes with the exogenous indole-3-acetic acid at 1 mg/l stimulates cell polarization and formation of a rhizoid protuberance. When auxin was added to the medium with triiodobenzoic acid, the inhibitory effect of the latter was eliminated. It has been proposed that the content of indole-3-acetic acid in the ambient medium is a key factor in the induction of polarity of the F. vesiculosus zygotes.
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Polevoi, V.V., Tarakhovskaya, E.R., Maslov, Y.I. et al. Role of Auxin in Induction of Polarity in Fucus vesiculosus Zygotes. Russian Journal of Developmental Biology 34, 360–364 (2003). https://doi.org/10.1023/B:RUDO.0000007890.08452.c4
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DOI: https://doi.org/10.1023/B:RUDO.0000007890.08452.c4